MUT antibody (N-Term)
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- Target See all MUT Antibodies
- MUT (Methylmalonyl Coenzyme A Mutase (MUT))
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Binding Specificity
- AA 33-65, N-Term
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MUT antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Methylmalonyl-CoA mutase, mitochondrial(MUT) detection. Tested with WB, IHC-P in Human,Rat.
- Sequence
- LHQQQPLHPE WAALAKKQLK GKNPEDLIWH TPE
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Methylmalonyl-CoA mutase, mitochondrial(MUT) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: methylmalonyl-CoA mutase
Protein Name: Methylmalonyl-CoA mutase, mitochondrial - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human MUT (33-65aa LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE), different from the related mouse sequence by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product MUT Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- MUT (Methylmalonyl Coenzyme A Mutase (MUT))
- Alternative Name
- MUT (MUT Products)
- Synonyms
- MCM antibody, D230010K02Rik antibody, Mcm antibody, methylmalonyl-CoA mutase antibody, methylmalonyl CoA mutase antibody, methylmalonyl-Coenzyme A mutase antibody, MUT antibody, mut antibody, Mut antibody
- Background
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Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.
Synonyms: MCM | Mut | P22033 - Gene ID
- 4594
- UniProt
- P22033
- Pathways
- Monocarboxylic Acid Catabolic Process
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