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Acetylated Lysine antibody

Details for Product No. ABIN488677, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine), Bird (Avian)
26
2
1
1
1
1
1
Host
Mouse
25
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
28
27
25
25
20
6
1
1
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Immunogen acetylated KLH
Clone 7F8
Isotype IgG1
Specificity The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
Predicted Reactivity higher plants
Purification affinity purified
Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Pathways
Application Notes Recommended Dilution needs to be determined for each application.
Comment

1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system

Restrictions For Research Use only
Format Liquid
Buffer protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 %glycerol at concentration 1 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Storage -20 °C
Background publications Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).