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Acetylated Lysine antibody

WB, ELISA, IP, IHC, IF, ICC Host: Mouse Monoclonal 7F8 unconjugated
Catalog No. ABIN488677
  • Target See all Acetylated Lysine products
    Acetylated Lysine
    Reactivity
    Please inquire
    Host
    • 29
    • 21
    Mouse
    Clonality
    • 27
    • 22
    Monoclonal
    Conjugate
    • 21
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Acetylated Lysine antibody is un-conjugated
    Application
    • 49
    • 38
    • 34
    • 22
    • 11
    • 11
    • 11
    • 11
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
    Cross-Reactivity (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Predicted Reactivity
    higher plants
    Purification
    affinity purified
    Immunogen
    acetylated KLH
    Clone
    7F8
    Isotype
    IgG1
  • Application Notes
    1: 100 (IHC), 1: 1000 (WB), for other assays optimal dilution has to be determined by end user
    Comment

    1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 %glycerol at concentration 1 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Storage
    -20 °C
    Storage Comment
    store at -20°C, avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    Acetylated Lysine
    Abstract
    Acetylated Lysine Products
    Synonyms
    C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, KRAS proto-oncogene, GTPase antibody, KRAS antibody
    Target Type
    Chemical
    Background
    Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
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