P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) antibody (FITC)

Antigen: P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP)

Synonyms: CD62, GRMP, PSEL, CD62P, GMP140, LECAM3, PADGEM, FLJ45155, Grmp, MGC129336, MGC129337, P-selectin, PSELECT, MGC124632, SELP

Clonality: Polyclonal

Host: Chicken

Reactivity: Human, Pig (Porcine)

Conjugate: FITC

Application: Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN488687

Additional Information

Alternative name: P-selectin (CD62P)

Immunogen: recombinant human P-selectin P16109

Format: Liquid

Description: Platelets or thrombocytes are the cell fragments circulating in the blood that areinvolved in the cellular mechanisms of primary hemostasis leading to theformation of blood clots. Dysfunction or low levels of platelets predisposes tobleeding, while high levels, although usually asymptomatic, may increase the riskof thrombosis. Platelets are produced in the bone marrow, the progenitor cell forplatelets is the megakaryocyte. It is about twelve times larger than an erythrocyte,possesses a lobed nucleus and sheds platelets into the circulation. Alternativeprotein names include: CD62P, GMP140, GMRP, Granule membrane protein 140,Granulocyte membrane protein, GRMP, LECAM 3, LECAM3, Leukocyteendothelial cell adhesion molecule 3, PADGEM, Platelet activation dependentgranule external membrane protein, PSEL, SELP

Specificity: Predicted reactivity: horse.

Application Details

Application Notes: Recommended Dilution 1: 1000 (WB), Flow cytometry: Suitable for detection of platelet activation by flowcytometry. Blood samples were collected in 5 mL sodium citrate tubes (367704,Becton Dickinson, Rutherford, NJ). Platelet-rich plasma was isolated bycentrifugation at room temperature. 5 mL platelet-rich plasma was added topolystyrene tubes containing 100 mL HEPES-buffer (137 mmol/L NaCl, 2.7mmol/L KCl, 1 mmol/L MgCl2 , 5.6 mmol/L glucose, 1 g/L bovine serum albuminand 20 mmol/L HEPES, pH 7.4) and 10 mL FITC labelled chicken antibody. Thesamples were incubated for 10 minutes at room temperature and were thendiluted and fixed with 1000 mL ice-cold PBS (0.02 mol/L Na2HPO4, 0.15 mol/LNaCl, 0.02% NaN3 , pH 7.2), containing 1 % p-formaldehyde. No washing stepswere used. The samples were analyzed utilising an Epics Profile XL-MCLcytometer (Coulter Electronics, Hialeah, FL). Data processing from 5,000 plateletswas carried out with the XL software (Coulter Electronics). Additional Information: the IgY fraction is isolated by a two-step PEG precipitation procedure followed byammonium sulphate precipitation, labelled with fluorescein.

Buffer: Total IgY fraction at 0.7 mg/ml 0.9 % NaCl, 0.1 % sodiumazide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Restrictions: For Research Use only