Ly-6C antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (7B10) |
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Host
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Reactivity
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Conjugate
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Application
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 Alternatives Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
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| Catalog no. |
ABIN488796 |
| Quantity |
0.5 mg (0.5 mg/ml) |
| Price |
243.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 10 Business Days |
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Isotype
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IgM
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Clone
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7B10
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Specificity
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Hamster Anti-Mouse Ly-6C recognizes Mouse Ly-6C . This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution: The Ly-6C antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested.
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Application Notes
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IHC (Frozen), FC Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 1.0 g/ml. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Ly-6C . A suggested method would be to stain cells expressing Ly-6C with Anti-Mouse Ly-6C at =1.0 g per 1.0 X 106 cells in a 100 l total staining volume, followed by UltraAvidin- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104). Each Investigator should determine their own optimal working dilution for specific applications.
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Concentration
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0.5 mg/ml
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Buffer
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This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
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Storage
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This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "Ly-6C", type "Antibodies"
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Hosts
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Rat (19), Armenian Hamster (4), Mouse (1)
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Reactivities
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Mouse (Murine) (24)
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Applications
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Flow Cytometry (FACS) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunohistochemistry (IHC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), Cytotoxicity Test (CyTox) (1), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Functional Studies (Func) (1)
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Conjugates
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Biotin (4), FITC (4), PE (3), APC (2), RPE (2)
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