FZD4 antibody (Frizzled Family Receptor 4) (AA 1-180) (Biotin)

Details for Product anti-FZD4 Antibody No. ABIN4898976, Supplier: Log in to see
Antigen
  • CG4626
  • DFz4
  • Dfz4
  • Dm Fz4
  • Dmel\\CG4626
  • Fz4
  • anon-WO0170980.10
  • anon-WO0170980.11
  • fz1
  • zg01
  • CD344
  • EVR1
  • FEVR
  • FZD4S
  • Fz-4
  • FzE4
  • GPCR
  • hFz4
  • frizzled4
  • fz4
  • FZ-4
  • frizzled 4
  • frizzled homolog 4
  • frizzled-4
  • frizzled family receptor 4
  • frizzled homolog 4 (Drosophila)
  • fz4
  • fzd4
  • Tsp_10376
  • FZD4
  • Fzd4
Alternatives
anti-Human FZD4 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 1-180
27
22
18
15
13
10
9
8
4
4
4
3
3
3
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
141
107
53
8
7
7
7
6
5
5
5
4
3
2
2
1
Host
Rat
100
34
25
8
Clonality (Clone)
Monoclonal ()
Conjugate
This FZD4 antibody is conjugated to Biotin
9
8
5
5
5
4
4
4
4
4
4
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
95
52
37
15
13
13
12
8
4
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4, Met1-Glu180, Accession # Q61088
Clone 145901
Isotype IgG2a
Specificity Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.15 EU per 1 μg of the antibody by the LAL method.
Alternative Name Frizzled-4 (FZD4 Antibody Abstract)
Background Alternative Names: CD344, CD344 antigen, EVR1, exudative vitreoretinopathy 1, FEVR, frizzled (Drosophila) homolog 4, frizzled homolog 4 (Drosophila), frizzled-4, Frizzled4, Fz-4, FZD4, FZD4S, FzE4, GPCR, hFz4, MGC34390, WNT receptor frizzled-4
Gene ID 8322
UniProt Q61088
Pathways WNT Signaling, Hormone Transport, Sensory Perception of Sound
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
Restrictions For Research Use only
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Product cited in: Despeaux, Chicanne, Rouer, De Toni-Costes, Bertrand, Mansat-De Mas, Vergnolle, Eaves, Payrastre, Girault, Racaud-Sultan: "Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered Wnt signaling." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 8, pp. 1597-610, 2012 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

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