Interleukin 33 antibody (IL33) (AA 109-266)

Details for Product anti-IL33 Antibody No. ABIN4899216, Supplier: Log in to see
Antigen
  • IL33
  • C9orf26
  • DVS27
  • IL1F11
  • NF-HEV
  • NFEHEV
  • RP11-575C20.2
  • 9230117N10Rik
  • Il-33
  • Il1f11
  • RGD1311155
  • interleukin 33
  • interleukin 33-like
  • interleukin-33-like
  • IL33
  • Il33
  • LOC100356081
  • LOC100518643
Alternatives
anti-Human Interleukin 33 antibody for Western Blotting
Epitope
AA 109-266
68
26
20
14
12
7
6
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
290
105
45
8
4
3
2
2
Host
Goat
188
133
23
23
4
3
2
1
1
Clonality
Polyclonal
Conjugate
This Interleukin 33 antibody is un-conjugated
36
19
14
14
8
7
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
278
215
88
60
45
23
21
20
16
14
13
11
7
5
5
4
3
3
2
2
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen E. coli-derived recombinant mouse IL-33, Ser109-Ile266, Accession # Q8BVZ5
Isotype IgG
Specificity Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2 % cross-reactivity with recombinant human IL-33 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Alternative Name IL-33 (IL33 Antibody Abstract)
Background Alternative Names: C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL1F11, IL-1F11, IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin-33, NFHEV, NF-HEV, NF-HEVNFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2
Gene ID 90865
UniProt Q8BVZ5
Research Area Angiogenesis, Cytokines, Cardiovascular, Innate Immunity, Hormones, Hypertrophy
Pathways Production of Molecular Mediator of Immune Response
Application Notes Western Blot 0.4 μg/mL
Immunohistochemistry 5-15 μg/mL
Neutralization 10-50 ng/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Neutralization (Neut) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) Proliferation Induced by IL-33 and Neutralization by Human IL-33 Antibody. Recombinan...
ELISA image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) IL-33 in Mouse Spleen. IL-33 was detected in immersion fixed frozen sections of mouse...
Flow Cytometry (FACS) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) Detection of IL-33 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were tre...
Immunocytochemistry (ICC) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) IL-33 in bEnd.3 Mouse Cell Line. IL-33 was detected in immersion fixed bEnd.3 mouse e...
Western Blotting (WB) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) Detection of Mouse IL-33 by Western Blot. Western blot shows lysates of HEK293T human...
Neutralization (Neut) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) Proliferation Induced by IL-33 and Neutralization by Human IL-33 Antibody. Recombinan...
Flow Cytometry (FACS) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) Detection of IL-33 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were tre...
Western Blotting (WB) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) Detection of Mouse IL-33 by Western Blot. Western blot shows lysates of HEK293T human...
Immunocytochemistry (ICC) image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) IL-33 in bEnd.3 Mouse Cell Line. IL-33 was detected in immersion fixed bEnd.3 mouse e...
ELISA image for anti-Interleukin 33 (IL33) (AA 109-266) antibody (ABIN4899216) IL-33 in Mouse Spleen. IL-33 was detected in immersion fixed frozen sections of mouse...
Product cited in: Athari, Poirier, Biton, Semerano, Hervé, Raffaillac, Lemeiter, Herbelin, Girard, Caux, Boissier, Bessis: "Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33." in: Arthritis research & therapy, Vol. 18, Issue 1, pp. 143, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Mahmutovic Persson, Akbarshahi, Menzel, Brandelius, Uller: "Increased expression of upstream TH2-cytokines in a mouse model of viral-induced asthma exacerbation." in: Journal of translational medicine, Vol. 14, pp. 52, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Aguiniga, Yaggie, Schaeffer, Klumpp: "Lipopolysaccharide Domains Modulate Urovirulence." in: Infection and immunity, Vol. 84, Issue 11, pp. 3131-3140, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Liu, Tai, Achanta, Kaelberer, Caceres, Shao, Fang, Jordt: "IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 47, pp. E7572-E7579, 2016 (PubMed). Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Denney, Byrne, Shea, Buckley, Pease, Herledan, Walker, Gregory, Lloyd: "Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function." in: Immunity, Vol. 43, Issue 5, pp. 945-58, 2015 (PubMed). Method employed by authors: ELISA (Capture) (ELISA (Capture)) (Sample species: Mouse (Murine)).

Kim, Morita, Kobayashi, Eisenbarth, Lee, Elias, Eynon, Flavell: "AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 22, pp. E2891-9, 2015 (PubMed). Method employed by authors: ELISA (Capture) (ELISA (Capture)) (Sample species: Mouse (Murine)).

Palomo, Reverchon, Piotet, Besnard, Couturier-Maillard, Maillet, Tefit, Erard, Mazier, Ryffel, Quesniaux: "Critical role of IL-33 receptor ST2 in experimental cerebral malaria development." in: European journal of immunology, Vol. 45, Issue 5, pp. 1354-65, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Mager, Riether, Schürch, Banz, Wasmer, Stuber, Theocharides, Li, Xia, Saito, Nakae, Baerlocher, Manz, McCoy, Macpherson, Ochsenbein, Beutler, Krebs: "IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms." in: The Journal of clinical investigation, Vol. 125, Issue 7, pp. 2579-91, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Sundnes, Pietka, Loos, Sponheim, Rankin, Pflanz, Bertelsen, Sitek, Hol, Haraldsen, Khnykin: "Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences." in: The Journal of investigative dermatology, Vol. 135, Issue 7, pp. 1771-80, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Millar, Gilchrist, Akbar, Reilly, Kerr, Campbell, Murrell, Liew, Kurowska-Stolarska, McInnes: "MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease." in: Nature communications, Vol. 6, pp. 6774, 2015 (PubMed). Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Liu, Kanaoka, Barrett, Feng, Garofalo, Lai, Buchheit, Bhattacharya, Laidlaw, Katz, Boyce: "Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 8, pp. 3537-45, 2015 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Bu, Zhang, Wang, Ren, Wen: "IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production." in: Journal of translational medicine, Vol. 13, pp. 381, 2015 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Polumuri, Jayakar, Shirey, Roberts, Perkins, Pitha, Vogel: "Transcriptional regulation of murine IL-33 by TLR and non-TLR agonists." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 1, pp. 50-60, 2012 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Paulissen, El Hour, Rocks, Guéders, Bureau, Foidart, Lopez-Otin, Noel, Cataldo: "Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 8, pp. 4135-43, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Nelson, Christmann, Werner, Metz, Trevor, Lowell, Steele: "IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 4, pp. 2372-81, 2011 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Byrne, Beaugie, OSullivan, Leighton, Halliday: "The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation." in: The American journal of pathology, Vol. 179, Issue 1, pp. 211-22, 2011 (PubMed). Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Ohno, Oboki, Morita, Kajiwara, Arae, Tanaka, Ikeda, Iikura, Akiyama, Inoue, Matsumoto, Sudo, Azuma, Okumura, Kamradt, Saito, Nakae: "Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation." in: PLoS ONE, Vol. 6, Issue 4, pp. e18404, 2011 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Enoksson, Lyberg, Möller-Westerberg, Fallon, Nilsson, Lunderius-Andersson: "Mast cells as sensors of cell injury through IL-33 recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 4, pp. 2523-8, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Hazlett, McClellan, Barrett, Huang, Zhang, Wu, van Rooijen, Szliter: "IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis." in: Investigative ophthalmology & visual science, Vol. 51, Issue 3, pp. 1524-32, 2010 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Ohno, Oboki, Kajiwara, Morii, Aozasa, Flavell, Okumura, Saito, Nakae: "Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7890-7, 2009 (PubMed). Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

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