SPARC antibody (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin)) (AA 18-303)

Details for Product anti-SPARC Antibody No. ABIN4899340, Supplier: Log in to see
Antigen
  • hm:zeh0062
  • AA517111
  • BM-40
  • ON
  • SPARC
  • secreted acidic cysteine rich glycoprotein
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • SPARC protein
  • SPARC-like
  • sparc
  • Sparc
  • SPARC
  • MGC64258
  • LOC100855736
Alternatives
anti-Human SPARC antibody for Immunohistochemistry
Epitope
AA 18-303
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2
2
2
1
1
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Reactivity
Human
203
89
46
17
8
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2
2
1
1
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Host
Goat
146
81
34
6
3
Clonality
Polyclonal
Conjugate
This SPARC antibody is un-conjugated
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10
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2
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2
2
2
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2
1
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1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
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106
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64
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27
13
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Options
Supplier
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Supplier Product No.
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Immunogen Mouse myeloma cell line NS0-derived recombinant human SPARC/Osteonectin, Ala18-Ile303, Accession # P09486
Isotype IgG
Specificity Detects human SPARC/Osteonectin in direct ELISAs and Western blots. In direct ELISAs, approximately 25 % cross-reactivity with recombinant mouse SPARC is observed, and less than 3 % cross-reactivity with recombinant human SPARC L1 is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Alternative Name SPARC (SPARC Antibody Abstract)
Background Alternative Names: Basement-membrane protein 40, BM-40, ONcysteine-rich protein, osteonectin, Secreted protein acidic and rich in cysteine, secreted protein, acidic, cysteine-rich (osteonectin)
Gene ID 6678
UniProt P09486
Research Area Lineage Markers, Extracellular Matrix, Angiogenesis, Tyrosine Kinases
Pathways Autophagy
Application Notes Western Blot 2 μg/mL
Simple Western 20 μg/mL
Immunohistochemistry 5-15 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (ABIN4899340) anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody
Simple Western (SimWes) image for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (ABIN4899340) anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (Image 2)
Western Blotting (WB) image for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (ABIN4899340) anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (Image 3)
Simple Western (SimWes) image for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (ABIN4899340) anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (Image 4)
Western Blotting (WB) image for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (ABIN4899340) anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (ABIN4899340) anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody (Image 6)
Product cited in: Riabov, Yin, Song, Avdic, Schledzewski, Ovsiy, Gratchev, Llopis Verdiell, Sticht, Schmuttermaier, Schönhaber, Weiss, Fields, Simon-Keller, Pfister, Berlit, Marx, Arnold, Goerdt, Kzhyshkowska: "Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model." in: Oncotarget, Vol. 7, Issue 21, pp. 31097-110, 2016 (PubMed).

Riordan, Varma, West, Brown: "Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling." in: PLoS ONE, Vol. 10, Issue 7, pp. e0128975, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Aguilera, Rivera, Hur, Carbon, Toombs, Goldstein, Dellinger, Castrillon, Brekken: "Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma." in: Cancer research, Vol. 74, Issue 4, pp. 1032-44, 2014 (PubMed). Method employed by authors: ELISA (ELISA) (Sample species: Human).

Chen, Wang, Xi, Xue, He, Zhang, Zhao: "SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer." in: PLoS ONE, Vol. 7, Issue 8, pp. e42413, 2012 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, 2012 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Shen, Siu, Byrd, Edelmann, Patel, Ketchem, Mehlin, Arnett, Hasegawa: "Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31." in: Experimental cell research, Vol. 317, Issue 7, pp. 976-93, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kelly, Allport, Yu, Sinh, Sage, Gerszten, Weissleder: "SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration." in: Journal of leukocyte biology, Vol. 81, Issue 3, pp. 748-56, 2007 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

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