EPH Receptor A2 antibody (EPHA2) (AA 25-534)

Details for Product anti-EPHA2 Antibody No. ABIN4899912, Supplier: Log in to see
Antigen
  • EPHA2
  • epha2
  • MGC89505
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • EPH receptor A2
  • EphA2
  • epoxide hydrolase
  • Eph receptor A2
  • EPHA2
  • epha2
  • ephA2
  • Epha2
Alternatives
anti-Human EPH Receptor A2 antibody for Flow Cytometry
Epitope
AA 25-534
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Reactivity
Human
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172
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This EPH Receptor A2 antibody is un-conjugated
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Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Mouse myeloma cell line NS0-derived recombinant human EphA2, Gln25-Asn534, Accession # P29317
Clone 371805
Isotype IgG2a
Specificity Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse EphA4, A5, A6, A7, A8, or recombinant rat EphB1 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Alternative Name EphA2 (EPHA2 Antibody Abstract)
Background Alternative Names: ARCC2, EC 2.7.10, EC 2.7.10.1, Eck, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, EphA2, ephrin type-A receptor 2, Epithelial cell kinase, Myk2, Sek2, soluble EPHA2 variant 1, Tyrosine-protein kinase receptor ECK
Gene ID 1969
UniProt P29317
Pathways RTK Signaling
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) EphA2 in A431 Human Cell Line. EphA2 was detected in immersion fixed A431 human epith...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial c...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial c...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) EphA2 in A431 Human Cell Line. EphA2 was detected in immersion fixed A431 human epith...
Product cited in: Liao-Chan, Daine-Matsuoka, Heald, Wong, Lin, Cai, Lai, DAlessio, Theunissen: "Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124708, 2015 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Park, Park, Kim, Pyo, Kimura, Sathirachinda, Choy, Min, Gambhir, Hong: "Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132976, 2015 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Yeast).

de Cárcer, Escobar, Higuero, García, Ansón, Pérez, Mollejo, Manning, Meléndez, Abad-Rodríguez, Malumbres: "Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression." in: Molecular and cellular biology, Vol. 31, Issue 6, pp. 1225-39, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

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