IL17A antibody (Interleukin 17A) (AA 20-155)

Details for Product anti-IL17A Antibody No. ABIN4900592, Supplier: Log in to see
Antigen
  • Il17
  • IL17A
  • CTLA8
  • IL-17
  • IL-17A
  • IL17
  • Ctla-8
  • Ctla8
  • CTLA-8
  • ChIL-17
  • IL-17F
  • interleukin 17A
  • interleukin 17A-like
  • interleukin 17F
  • Il17a
  • IL17A
  • LOC100339322
  • IL17F
Alternatives
anti-Human IL17A antibody for Biochemical Assay
Epitope
AA 20-155
26
24
13
12
11
10
10
7
7
6
4
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
374
134
42
7
7
5
3
3
2
2
Host
Goat
248
135
77
39
3
3
Clonality
Polyclonal
Conjugate
This IL17A antibody is un-conjugated
56
32
27
20
16
15
15
12
11
11
10
7
7
7
7
7
5
5
5
4
4
4
4
4
4
3
2
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
270
229
194
43
29
20
19
16
8
8
7
7
6
5
4
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Immunogen E. coli-derived recombinant human IL-17, Ile20-Ala155, Accession # Q16552
Isotype IgG
Specificity Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 60 % cross-reactivity with recombinant canine IL-17 is observed, approximately 10 % cross-reactivity with recombinant human (rh) IL-17F and with recombinant mouse IL-17 is observed, and less than 1 % cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D, and rhIL-17E is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Alternative Name IL-17/IL-17A (IL17A Antibody Abstract)
Background Alternative Names: CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A
Gene ID 3605
UniProt Q16552
Application Notes Western Blot 1 μg/mL
Immunohistochemistry 1-15 μg/mL
Immunoprecipitation 3 μg/100 μg cell lysate
Neutralization 0.02-0.12 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Neutralization (Neut) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) IL-6 Secretion Induced by IL-17 and Neutralization by Human IL-17 Antibody.
Flow Cytometry (FACS) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) Detection of IL-17 in Human PBMCs by Flow Cytometry.
Immunohistochemistry (IHC) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) IL-17/IL-17A in Human Tonsil. IL-17/IL-17A was detected in immersion fixed paraffin-e...
Immunoprecipitation (IP) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) Immunoprecipitation of Human IL-17.
Western Blotting (WB) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) Detection of Human IL-17 by Western Blot.
Western Blotting (WB) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) Detection of Human IL-17 by Western Blot.
Flow Cytometry (FACS) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) Detection of IL-17 in Human PBMCs by Flow Cytometry.
Immunohistochemistry (IHC) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) IL-17/IL-17A in Human Tonsil. IL-17/IL-17A was detected in immersion fixed paraffin-e...
Neutralization (Neut) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) IL-6 Secretion Induced by IL-17 and Neutralization by Human IL-17 Antibody.
Immunoprecipitation (IP) image for anti-IL17A antibody (Interleukin 17A) (AA 20-155) (ABIN4900592) Immunoprecipitation of Human IL-17.
Product cited in: Millar, Akbar, Campbell, Reilly, Kerr, McLean, Frleta-Gilchrist, Fazzi, Leach, Rooney, Crowe, Murrell, McInnes: "IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy." in: Scientific reports, Vol. 6, pp. 27149, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Fattahi, Brandsma, Lodewijk, Reinders-Luinge, Postma, Timens, Hylkema, Ten Hacken: "Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161433, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Mariuzzi, Domenis, Orsaria, Marzinotto, Londero, Bulfoni, Candotti, Zanello, Ballico, Mimmi, Calcagno, Marchesoni, Di Loreto, Beltrami, Cesselli, Gri: "Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 96, Issue 9, pp. 959-71, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Nussbaumer-Ochsner, Stolk, Ferraz da Silva, van Schadewijk, de Jeu, Prins, Mauad, Rabe, Hiemstra: "Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?" in: PLoS ONE, Vol. 10, Issue 6, pp. e0129426, 2015 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Martin, Kesselring, Saidou, Brunner, Schiechl, Mouris, Wege, Rümmele, Schlitt, Geissler, Fichtner-Feigl: "RORγt(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice." in: European journal of immunology, Vol. 45, Issue 6, pp. 1667-79, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Punt, Houwing-Duistermaat, Schulkens, Thijssen, Osse, de Kroon, Griffioen, Fleuren, Gorter, Jordanova: "Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer." in: Molecular cancer, Vol. 14, pp. 71, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Chang, Al-Alwan, Alshakfa, Audusseau, Mogas, Chouiali, Nair, Baglole, Hamid, Eidelman: "Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD." in: Respiratory research, Vol. 15, pp. 145, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Liu, Jin, Zhang, Lin, Chen, Sun, Zhang, Zhang, Yu: "Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients." in: PLoS ONE, Vol. 9, Issue 9, pp. e106834, 2014 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Keijsers, Hendriks, van Erp, van Cranenbroek, van de Kerkhof, Koenen, Joosten: "In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORγt and IL-17." in: The Journal of investigative dermatology, Vol. 134, Issue 5, pp. 1276-84, 2014 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Hendriks, van der Velden, Wolberink, Seyger, Schalkwijk, Zeeuwen, de Jong, Pasch, van Erp, van de Kerkhof: "The effect of adalimumab on key drivers in the pathogenesis of psoriasis." in: The British journal of dermatology, Vol. 170, Issue 3, pp. 571-80, 2014 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Shimizu, Tasaki, Sano, Ju, Nishihara, Oishi, Koga, Kawai, Kanda: "Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier." in: PLoS ONE, Vol. 9, Issue 3, pp. e92872, 2014 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Human).

Todd, Simpson, Estis, Torres, Wub: "Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays." in: Cytokine, Vol. 64, Issue 3, pp. 660-5, 2013 (PubMed). Method employed by authors: ELISA (ELISA) (Sample species: Human).

Lotti, Jarrar, Pai, Hitomi, Lathia, Mace, Gantt, Sukhdeo, DeVecchio, Vasanji, Leahy, Hjelmeland, Kalady, Rich: "Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A." in: The Journal of experimental medicine, Vol. 210, Issue 13, pp. 2851-72, 2013 (PubMed). Method employed by authors: Immunofluorescence (IF) (Sample species: Human).

Truchetet, Brembilla, Montanari, Lonati, Raschi, Zeni, Fontao, Meroni, Chizzolini: "Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement." in: Arthritis and rheumatism, Vol. 65, Issue 5, pp. 1347-56, 2013 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Xing, Yang, Yang, Wei, Zhu, Gao, Li: "IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway." in: PLoS ONE, Vol. 8, Issue 12, pp. e85032, 2013 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Human).

Gelderblom, Weymar, Bernreuther, Velden, Arunachalam, Steinbach, Orthey, Arumugam, Leypoldt, Simova, Thom, Friese, Prinz, Hölscher, Glatzel, Korn, Gerloff, Tolosa, Magnus: "Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke." in: Blood, Vol. 120, Issue 18, pp. 3793-802, 2012 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Fontao, Brembilla, Masouyé, Kaya, Prins, Dupin, Saurat, Chizzolini, Piguet: "Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin." in: The British journal of dermatology, Vol. 166, Issue 3, pp. 687-9, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Droeser, Zlobec, Kilic, Güth, Heberer, Spagnoli, Oertli, Tapia: "Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers." in: BMC cancer, Vol. 12, pp. 134, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Liu, Lee, McManus, Choy: "Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 3, pp. 1544-50, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Lapinski, Kwon, Lubeck, Wilkinson, Srinivasan, Sevick-Muraca, King: "RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice." in: The Journal of clinical investigation, Vol. 122, Issue 2, pp. 733-47, 2012 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Human).