ADD2 antibody

Catalog No. ABIN4902845

Product Details anti-ADD2 Antibody

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADD2 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]

Purification: Affinity purification

Immunogen: Recombinant protein of human ADD2.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for ADD2

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Alternative Name: ADD2 (ADD2 Products)

Synonyms: si:dkey-202n14.5 antibody, ADDB antibody, 2900072M03Rik antibody, add97 antibody, beta-ADD antibody, adducin 2 antibody, adducin 2 (beta) antibody, ADD2 antibody, add2 antibody, Add2 antibody

Gene ID: 119

UniProt: P35612

Pathways: Regulation of Actin Filament Polymerization