Nebulette antibody (AA 181-190)

Catalog No. ABIN490371

Product Details anti-Nebulette Antibody

Target: Nebulette (NEBL)

Binding Specificity: AA 181-190

Reactivity: Human, Mouse, Chicken, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Nebulette antibody is un-conjugated

Application: ELISA

Specificity: Human NEBL. This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1, NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Panda, Opossum, Chicken (100%) Xenopus (90%) Lizard, Pufferfish, Ant, Phytophthora, Ajellomyces, Stigmatella, Salpingoeca (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ELQRLKRTQE from an internal region of human NEBL (NP_998734.1, NP_001166955.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Panda, Opossum, Chicken (100%), Xenopus (90%), Lizard, Pufferfish, Ant, Phytophthora, Ajellomyces, Stigmatella, Salpingoeca (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:4000)

Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx 30 kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/mL antibody staining. This protein has a calculated MW of 31.2 kDa according to NP_998734.1. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Nebulette

Target: Nebulette (NEBL)

Alternative Name: NEBL LASP2 (NEBL Products)

Synonyms: LASP-2 antibody, zgc:113144 antibody, LASP2 antibody, LNEBL antibody, 1200007O21Rik antibody, A630080F05Rik antibody, BB140644 antibody, D830029A09Rik antibody, Lnebl antibody, nebulette antibody, nebulette S homeolog antibody, Nebl antibody, NEBL antibody, nebl antibody, nebl.S antibody

Background: Name/Gene ID: NEBL

Synonyms: NEBL, Actin-binding Z-disk protein, LASP2, LIM-nebulette, LNEBL, Nebulette

Gene ID: 10529

NCBI Accession: NP_998734, NP_001166955

UniProt: O76041