CHKB antibody (AA 322-337)

Catalog No. ABIN490395

Product Details anti-CHKB Antibody

Target: CHKB (Choline Kinase beta (CHKB))

Binding Specificity: AA 322-337

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This CHKB antibody is un-conjugated

Application: ELISA

Specificity: Human CHKB / CHKL. This antibody is expected to recognize isoform a (NP_005189.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Elephant, Panda (94%) Dog, Rabbit, Pig (88%) Bat, Bovine, Horse (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KGETLSQEEQRKLEED from an internal region of human CHKB / CHKL (NP_005189.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Elephant, Panda (94%), Dog, Rabbit, Pig (88%), Bat, Bovine, Horse (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:2000)

Usage: Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Preliminary experiments gave an approx 38  kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3  kDa according to NP_005189.2. The 38  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CHKB

Target: CHKB (Choline Kinase beta (CHKB))

Alternative Name: CHKB / CHKL (CHKB Products)

Synonyms: CHETK antibody, CHKL antibody, CK antibody, CKB antibody, CKEKB antibody, EK antibody, EKB antibody, MDCMC antibody, Chetk antibody, Chkl antibody, Ck antibody, Ck/Ek antibody, Ck/Ek-beta antibody, Ckb antibody, Ckekb antibody, Ek antibody, fc21g05 antibody, si:ch211-218c17.2 antibody, wu:fc21g05 antibody, choline kinase beta antibody, CHKB antibody, Chkb antibody, chkb antibody

Background: Name/Gene ID: CHKB

Synonyms: CHKB, Choline kinase beta, Choline kinase-like, CK, CHETK, CKEKB, CHKL, Choline/ethanolamine kinase, Ethanolamine kinase beta, Ethanolamine kinase, MDCMC, Choline kinase-like protein, EK, EKB

Gene ID: 1120

NCBI Accession: NP_005189

UniProt: Q9Y259