UPF3A antibody (AA 233-245)

Catalog No. ABIN490593

Product Details anti-UPF3A Antibody

Target: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Binding Specificity: AA 233-245

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This UPF3A antibody is un-conjugated

Application: ELISA

Specificity: Human UPF3A. This antibody is expected to recognize both isoforms (NP_075387.1, NP_542418.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Bat (92%) Marmoset, Opossum, Lizard (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KETDKQKKIAEKE from an internal region of human UPF3A (NP_075387.1, NP_542418.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Bat (92%), Marmoset, Opossum, Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 48  kDa band in lysates of cell lines HeLa and Kelly after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51  kDa according to NP_542418.1 and the reported sizes of 65 to 70  kDa from different sources. The 48  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for UPF3A

Target: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Alternative Name: UPF3A / UPF3 (UPF3A Products)

Synonyms: 2600001C03Rik antibody, 4930546M19Rik antibody, RENT3A antibody, UPF3 antibody, UPF3A antibody, HUPF3A antibody, si:dkey-21o13.6 antibody, UPF3 regulator of nonsense transcripts homolog A (yeast) antibody, UPF3A, regulator of nonsense mediated mRNA decay antibody, Upf3a antibody, UPF3A antibody, upf3a antibody

Background: Name/Gene ID: UPF3A

Synonyms: UPF3A, HUPF3A, HUpf3, RENT3A, UPF3

Gene ID: 65110

NCBI Accession: NP_075387, NP_542418

UniProt: Q9H1J1