RDH5 antibody (AA 209-221)

Catalog No. ABIN490712

Product Details anti-RDH5 Antibody

Target: RDH5 (Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5))

Binding Specificity: AA 209-221

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This RDH5 antibody is un-conjugated

Application: ELISA

Specificity: Human RDH5.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster (92%) Elephant, Dog, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RTPVTNLESLEKT from an internal region of human RDH5 (NP_002896.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster (92%), Elephant, Dog, Bovine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 70  kDa and 40  kDa in Mouse Eye lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 35.0  kDa according to NP_002896.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RDH5

Target: RDH5 (Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5))

Alternative Name: RDH1 / RDH5 (RDH5 Products)

Synonyms: 9cRDH antibody, HSD17B9 antibody, RDH1 antibody, SDR9C5 antibody, RDHB antibody, MGC84134 antibody, RDH5 antibody, rdh1 antibody, hsd17b9 antibody, im:6901729 antibody, si:dkey-102c8.5 antibody, 9-cis antibody, AI987873 antibody, RDH4 antibody, cRDH antibody, retinol dehydrogenase 5 antibody, retinol dehydrogenase 1 antibody, retinol dehydrogenase 5 L homeolog antibody, retinol dehydrogenase 5 (11-cis/9-cis) antibody, RDH5 antibody, rdh1 antibody, rdh5.L antibody, rdh5 antibody, Rdh5 antibody

Background: Name/Gene ID: RDH5

Synonyms: RDH5, 11-cis retinol dehydrogenase, 9cRDH, 11-cis RDH, Retinol dehydrogenase 1, SDR9C5, HSD17B9, RDH1

Gene ID: 5959

NCBI Accession: NP_002896

UniProt: Q92781