KCNMB2 antibody (C-Term, N-Term)

Catalog No. ABIN492461

Product Details anti-KCNMB2 Antibody

Target: KCNMB2 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 2 (KCNMB2))

Binding Specificity: AA 1-41, C-Term, N-Term

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNMB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects ~27 kDa. No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4. Not yet validated for use in Mammalian brain.

Characteristics: Synonyms: BK channel subunit beta-2, Hbeta3, K(VCA)beta-2, Slo-beta-2, Charybdotoxin receptorsubunit beta-2, Maxi K channel subunit beta-2, Calcium-activated potassium channelsubunit beta-2, Calcium-activated potassium channel, subfamily M subunit beta-2

Purification: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of Mouse BKBeta2(accession number Q9CZM9).

Clone: S53-32

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of BK Beta2 in 10 μg of COS cell (lysate) transientlytransfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouseIgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KCNMB2

Target: KCNMB2 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 2 (KCNMB2))

Alternative Name: KCNMB2 (KCNMB2 Products)

Synonyms: KCNMB3 antibody, zgc:101842 antibody, KCNMB2 antibody, 2700049B16Rik antibody, 3110031N04Rik antibody, Kcmb2 antibody, potassium calcium-activated channel subfamily M regulatory beta subunit 2 antibody, potassium large conductance calcium-activated channel, subfamily M, beta member 2 antibody, KCNMB2 antibody, kcnmb2 antibody, Kcnmb2 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (3). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (4). This has major implications on other physiological processes in tissues other than the brain.Synonyms: BK channel subunit beta-2, BKbeta2, Calcium-activated potassium channel subfamily M subunit beta-2, Calcium-activated potassium channel subunit beta-2, Charybdotoxin receptor subunit beta-2, Hbeta2, Hbeta3, K(VCA)beta-2, Maxi K channel subunit beta-2, Slo-beta-2

Gene ID: 72413

UniProt: Q9CZM9