Kv3.4 antibody (AA 175-192)

Catalog No. ABIN492465

Product Details anti-Kv3.4 Antibody

Target: Kv3.4 (KCNC4) (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 4 (KCNC4))

Binding Specificity: AA 175-192

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Kv3.4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects ~70 kDa protein (100 kDa in brain due to glycosylation).

Characteristics: Synonyms: Potassium voltage-gated channel subfamily C member 4, Voltage-gated potassiumchannel subunit Kv3.4, KSHIIIC

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide amino acids 175-192 of Rat Kv3. 4 (accession number Q63734)AA Sequence: GDEAGDDERELALQRLGP

Clone: S72-16

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Kv3.4 in 10 μg of Rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Kv3.4

Target: Kv3.4 (KCNC4) (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 4 (KCNC4))

Alternative Name: KCNC4 (KCNC4 Products)

Synonyms: C1orf30 antibody, HKSHIIIC antibody, KSHIIIC antibody, KV3.4 antibody, AI850292 antibody, Kcr2-4 antibody, Kv3.4 antibody, potassium voltage-gated channel subfamily C member 4 antibody, potassium channel, voltage gated Shaw related subfamily C, member 4 antibody, potassium voltage gated channel, Shaw-related subfamily, member 4 antibody, KCNC4 antibody, kcnc4 antibody, Kcnc4 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Kv3.4, a member of the Shaw subfamily, may play important roles in maintaining normal function of the corneal epithelium (3). Kv3.4 is also over-expressed in the early stages of Alzheimer's disease, and therefore represents a novel therapeutic target for this disease (4).Synonyms: KSHIIIC, Potassium voltage-gated channel subfamily C member 4, Voltage-gated potassium channel subunit Kv3.4

Gene ID: 684516

UniProt: Q63734