SHANK3 antibody (AA 840-857)

Catalog No. ABIN492466

Product Details anti-SHANK3 Antibody

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Binding Specificity: AA 840-857

Reactivity: Rat, Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects ~190 kDa protein. No cross-reactivity against Shank1 or Shank2.

Characteristics: Synonyms: PROSAP2, PSAP2, SPANK-2, SPANK2, KIAA1650, SH3 and multiple ankyrin repeat domainsprotein 3, Proline-rich synapse-associated protein 2

Purification: Protein G Chromatography

Immunogen: Synthetic peptide amino acids 840-857 of Rat Shank3 (accession number Q9JLU4). AA Sequence: PEKLPGSLRKGIPRTKSV

Clone: S69-46

Isotype: IgG2b

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Shank3 in 10 μg of COS cell (lysate) transientlytransfected with Shank3 by colorimetric immunoblot analysis using Goat anti-mouseIgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: SHANK3 (SHANK3 Products)

Synonyms: DEL22q13.3 antibody, PROSAP2 antibody, PSAP2 antibody, SCZD15 antibody, SPANK-2 antibody, Prosap2 antibody, AI841104 antibody, Shank3b antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody, SHANK3 antibody, shank3 antibody, Shank3 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (3). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (4). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (5).Synonyms: KIAA1650, PROSAP2, PSAP2, Proline-rich synapse-associated protein 2, SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, SPANK2

Gene ID: 59312

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity