PUMA antibody (AA 76-170)

Catalog No. ABIN492517

Product Details anti-PUMA Antibody

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Binding Specificity: AA 76-170

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Recognizes Human and Rat PUMA. Other species not tested.

Characteristics: Synonyms: BBC3, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis

Purification: Immunoaffinity Chromotography

Immunogen: A recombinant protein corresponding to amino acids 76–170 of Human PUMA-alpha

Clone: 2A8F6

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: PUMA antibody can be used for detection of PUMA at 2.5-5 μg/mL. Positive Control: K562 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA (BBC3 Products)

Synonyms: BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody

Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity