PSMA antibody (FITC)

Catalog No. ABIN492597

Product Details anti-PSMA Antibody (FITC)

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with PSMA (100 kDa) on Flow Cytometry.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen,FOLH, NAALAD1, PSM, GCP2, NAALAdase, Glutamate carboxypeptidase II, Membraneglutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I,Pteroylpoly-gamma-glutamate carboxypeptidase, Folylpoly-gamma-glutamatecarboxypeptidase

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: LNCaP cell homogenate.

Clone: 107-1A4

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10 μg/mL (final concentration). Positive Control: LNCaP cell. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric analysis for for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Detach the cells from culture dish by using cell dissociation buffer2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN]. 3) Resuspend the cells with washing buffer (5x10e6 cells/mL). 4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 5) Add 20 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 6) Add 30 µL of the primary antibody at the concentration of as suggest in theAPPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at roomtemperature. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 8) Add 30 µL of 1: 100 FITC conjugated anti-mouse IgG diluted with the washing buffer. Mixwell and incubate for 15 minutes at room temperature. 9) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 10) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: LNCaP

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 1 % BSA and 0.09 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-PSMA antibody (FITC) (ABIN492597)

Gorges, Riethdorf, von Ahsen, Nastał Y, Röck, Boede, Peine, Kuske, Schmid, Kneip, König, Rudolph, Pantel: "Heterogeneous PSMA expression on circulating tumor cells: a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer." in: Oncotarget, Vol. 7, Issue 23, pp. 34930-41, (2018) (PubMed).

Target Details for PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Alternative Name: PSMA / FOLH1 (FOLH1 Products)

Synonyms: FGCP antibody, FOLH antibody, GCP2 antibody, GCPII antibody, NAALAD1 antibody, NAALAdase antibody, PSM antibody, PSMA antibody, mGCP antibody, mopsm antibody, Naalad antibody, putative N-acetylated-alpha-linked acidic dipeptidase antibody, folate hydrolase 1B antibody, folate hydrolase 1 antibody, folate hydrolase (prostate-specific membrane antigen) 1 antibody, LOC451185 antibody, FOLH1B antibody, FOLH1 antibody, Folh1 antibody

Background: PSMA is a type II transmembrane protein expressed at high levels in prostate cancer and in tumor-associated neovasculature. PSMA is also known as a glutamate carboxypeptidase II (EC 3.4.17.21) that hydrolyzes the neuropeptide, N-acetyl-L-aspartyl-L-glutamate, releasing glutamate, the dominant excitatory neurotransmitter/neuromodulator of the mammalian central nervous system. The ability of PSMA to liberate g-glutamate may allow the removal of glutamate residues from dietary folates, which are unable to be transported into cells in their poly-g-glutamate form. Overexpression of PSMA in prostate cancer cells may represent an advantageous adaptation that allows the uptake of folates required for rapid division. The exopeptidase activity exhibited by PSMA and its high-level overexpression in prostate cancer cells make it a potential target for selective anticancer therapy and prodrug activation.Synonyms: FOLH, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALAD1, NAALAdase, PSM, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase

Gene ID: 2346

UniProt: Q04609