Did you know that you can buy products from over 140 different suppliers from us?

Orexin A (OXA) antibody

Details for Product No. ABIN492717
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms orexin-A
Reactivity
Mouse (Murine), Rat (Rattus)
(52), (26), (25), (24), (24), (24)
Host
Rabbit
(51), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application
ELISA, Western Blotting (WB)
(30), (24), (14), (10), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN492717
346.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptides corresponding to unique epitope on Orexin-A.
Specificity This antibody detects a single band of approximately 16kDa in Western blot positive control samples for Orexin-A and several other tissues.
Purification Affinity Chromatography on immobilized antigen
Alternative Name Orexin-A
Background Orexin-A is a hypothalamic neuropeptide that belongs to the Orexin family (Orexin-A and Orexin-B) (1). Orexin neuropeptides -A and -B or Orexins are also known as Hypocretins. Orexin-A and –B are derived from the same precursor peptide (prepro-orexin) by proteolytic processing whose gene is located at human chromosome 17q21 (2). Orexins are produced by a small group of neurons located in the later hypothalamic and perifornical areas, with widespread projections to the entire neuroaxis (3). Orexinergic neurons of the lateral hypothalamus act as glucose sensors. Orexin signals hunger when blood glucose levels are low. Orexins also play a significant role in the regulation of sleep-wakefulness and body fluids and, in homeostatic regulation of energy metabolism, autonomic function and hormonal balance (4). Orexins mediate their effects by binding to two G-Protein-Coupled Receptors (OXR1 and OXR2). Orexin-A binds to both OXR1 and OXR2 with high affinity (5). Orexin binding to its associated receptor activates the hypoxia-inducible factor 1 (HIF-1) transcription factor in the hypothalamus which results in increased glucose uptake and higher glycolytic activity. Mice lacking orexins or their cognate receptors eat less compared with matched littermates that do not lack them and, display a sleep disorder that is similar to human narcolepsy (6). Therefore, orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid of most narcoleptic patients. Orexin-A is expressed in subthalamic nucleus, heart, lung, liver, kidney, pancreas, placenta and skeletal muscle. It is approximately a 16kDa protein (131 amino acids).
Alternate names: HCRT, Hypocretin, OX, PPORX, PPOX
Gene ID 25723
NCBI Accession NP_037311
UniProt O55232
Research Area Hormones
Application Notes Suitable for use in ELISA (< 1/10,000) and Western Blot (> 1/500). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.64-0.72 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20°C.
Expiry Date 12 months
Background publications Sakurai, Moriguchi, Furuya et al.: "Structure and function of human prepro-orexin gene." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17771-6, 1999 (PubMed).

Willie, Chemelli, Sinton et al.: "To eat or to sleep? Orexin in the regulation of feeding and wakefulness." in: Annual review of neuroscience, Vol. 24, pp. 429-58, 2001 (PubMed).

Hungs, Mignot: "Hypocretin/orexin, sleep and narcolepsy." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 23, Issue 5, pp. 397-408, 2001 (PubMed).

Ueta: "[Involvement of orexins/hypocretins in multiple physiological functions]." in: Journal of UOEH, Vol. 23, Issue 2, pp. 147-59, 2001 (PubMed).

Sikder, Kodadek: "The neurohormone orexin stimulates hypoxia-inducible factor-1 activity." in: Genes & development, Vol. 21, Issue 22, pp. 2995-3005, 2007 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN492717
346.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top