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Orexin (OX) antibody

Details for Product No. ABIN492717, Supplier: Log in to see
  • orexin-A
  • PPOX
  • NRCLP1
  • OX
  • hypocretin
  • hypocretin (orexin) neuropeptide precursor
  • Hcrt
  • HCRT
Mouse (Murine), Rat (Rattus)
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Immunogen Synthetic peptides corresponding to unique epitope on Orexin-A.
Specificity This antibody detects a single band of approximately 16 kDa in Western blot positive control samples for Orexin-A and several other tissues.
Cross-Reactivity (Details) Species reactivity (tested):Mouse and Rat.
Purification Affinity Chromatography on immobilized antigen
Alternative Name Orexin (OX Antibody Abstract)
Background Orexin-A is a hypothalamic neuropeptide that belongs to the Orexin family (Orexin-A and Orexin-B) (1). Orexin neuropeptides -A and -B or Orexins are also known as Hypocretins. Orexin-A and -B are derived from the same precursor peptide (prepro-orexin) by proteolytic processing whose gene is located at human chromosome 17q21 (2). Orexins are produced by a small group of neurons located in the later hypothalamic and perifornical areas, with widespread projections to the entire neuroaxis (3). Orexinergic neurons of the lateral hypothalamus act as glucose sensors. Orexin signals hunger when blood glucose levels are low. Orexins also play a significant role in the regulation of sleep-wakefulness and body fluids and, in homeostatic regulation of energy metabolism, autonomic function and hormonal balance (4). Orexins mediate their effects by binding to two G-Protein-Coupled Receptors (OXR1 and OXR2). Orexin-A binds to both OXR1 and OXR2 with high affinity (5). Orexin binding to its associated receptor activates the hypoxia-inducible factor 1 (HIF-1) transcription factor in the hypothalamus which results in increased glucose uptake and higher glycolytic activity. Mice lacking orexins or their cognate receptors eat less compared with matched littermates that do not lack them and, display a sleep disorder that is similar to human narcolepsy (6). Therefore, orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid of most narcoleptic patients. Orexin-A is expressed in subthalamic nucleus, heart, lung, liver, kidney, pancreas, placenta and skeletal muscle. It is approximately a 16 kDa protein (131 amino acids).Synonyms: HCRT, Hypocretin, OX, PPORX, PPOX
Gene ID 25723
NCBI Accession NP_037311
UniProt O55232
Research Area Hormones
Application Notes Suitable for use in ELISA (< 1/10,000) and Western Blot (> 1/500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.64-0.72 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Background publications Sikder, Kodadek: "The neurohormone orexin stimulates hypoxia-inducible factor-1 activity." in: Genes & development, Vol. 21, Issue 22, pp. 2995-3005, 2007 (PubMed).

Willie, Chemelli, Sinton, Yanagisawa: "To eat or to sleep? Orexin in the regulation of feeding and wakefulness." in: Annual review of neuroscience, Vol. 24, pp. 429-58, 2001 (PubMed).

Ueta: "[Involvement of orexins/hypocretins in multiple physiological functions]." in: Journal of UOEH, Vol. 23, Issue 2, pp. 147-59, 2001 (PubMed).

Hungs, Mignot: "Hypocretin/orexin, sleep and narcolepsy." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 23, Issue 5, pp. 397-408, 2001 (PubMed).

Sakurai, Moriguchi, Furuya, Kajiwara, Nakamura, Yanagisawa, Goto: "Structure and function of human prepro-orexin gene." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17771-6, 1999 (PubMed).