Chemokine (C-C Motif) Receptor 1 (CCR1) (AA 7-24) antibody

Antigen: Chemokine (C-C Motif) Receptor 1 (CCR1)

Synonyms: CKR1, CD191, CKR-1, HM145, CMKBR1, MIP1aR, SCYAR1

Epitope: AA 7-24

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN492850

Additional Information

Characteristics: Synonyms: C-C chemokine receptor type 1, C-C CKR-1, CC-CKR-1, CCR-1, CMKBR1, CMKR1, SCYAR1,HM145, LD78 receptor, Macrophage inflammatory protein 1-alpha receptor, MIP1 alphaReceptor, RANTES-R

Alternative name: CD191 / CCR1

Gene ID: 1230

Swiss-Prot: P32246

Immunogen: Synthetic peptide for CCR1 receptor aa 7-24AA Sequence: tpd ydt tte fdy gda tpc

Cross-Reactivity: Cow (Bovine), Human, Monkey, Mouse (Murine), Rat (Rattus)

Description: Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators andchemo-attractants for leukocyte subpopulations and other nonhemopoietic cells. Chemokine receptors (CCR) belong to the superfamily of G protein-coupled receptors(GPCR), which regulate the trafficking and activation of leukocytes, and operate ascoreceptors in the entry of HIV-1 and directing the proliferation and migration of immatureneurons, glia and their precursors (1). Furthermore, chemokine receptors participate in theetiology and progression of various brain disorders, including AIDS dementia,neuro-inflammatory disease and neuroplasia, making them important potentialtherapeutic targets in these cases. Induction of chemokines and infiltration of chemokinereceptor-bearing cells has also been shown in a variety of animal models of renal diseases,as well as in human diseases and allograft rejection (2). Activation of naïve T cells by specific antigen and cytokines, up-regulate cell adhesionmolecules (CAM) as well as chemokine receptors on their surface, which directs them tomigrate towards the inflamed tissues. Several subtypes of CCR have been characterized(Table1). CCR1 has been implicated in IL8 induced chemotaxis of melanoma cells. Thenonresponsive WM35 melanoma cells when stimulated by TNF express higher levels ofCXCR1 and exhibit chemotactic towards interleukin-8 (3). Stimulation of CXCR1 also inducefocal adhesion kinase (FAK) phosphorylation, that regulate the chemotactic activities ofthe migrating cells (4). On the contrary, glucocorticoid/dexamethasone has potentanti-inflammatory effects, yet it up-regulates expression of CXCR4 but not that of CXCR1and CXCR3 (4). The CXCR receptor protein is approximately 45-55 kDa (405 amino acids) protein which has7 transmembrane domains, characteristic of G-protein coupled receptors. The all CXCRsequence analyses suggest the presence of 7 transmembrane domains, a characteristicfeature of GPCR. The protein has putative N-glycosylation sites near the extra cellularN-terminal end of the proteins. The protein has a large 3rd intra cellular loop whichinteracts with G-proteins. The short carboxy terminal is intracellular and has putativepost-translational sites.

Specificity: This antibody detects CD191 / CCR1 at aa 7-24. Species Reactivity: Tested: Human, bovine, monkey, mouse, rat

Application Details

Application Notes: Immunohistochemistry on frozen sections. ELISA. Western blot: > 1: 500. IMM and i. p pull-down assays: > 1: 250. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 - 0.6 mg/ml (lot specific)

Purification: Aff - Purified

Buffer: Stabilization buffer

Storage: Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only