Forkhead Box P3 (FOXP3) (AA 229-250) antibody

Details for Product No. ABIN492998
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Antigen
Synonyms foxp3, FOXP3, AIID, DIETER, IPEX, PIDX, XPID, JM2, scurfin, sf, RGD1562112
Epitope
AA 229-250
(72), (18), (7), (7), (7), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Monkey, Mouse (Murine)
(199), (136), (60), (15), (15), (13), (12), (6)
Host
Rabbit
(160), (60), (43), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(18), (16), (15), (9), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
Immunoprecipitation (IP), Western Blotting (WB)
(176), (90), (81), (54), (43), (20), (9), (9), (8), (8), (7), (7), (3), (3)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN492998
346.50 $
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Immunogen Synthetic peptides selected from forkhead/winged helix transcription factor 3 (Fox3p) corresponding to amino acids 229-250 of mouse protein
Sequence AQC LLQ REV VQS LEQ QLE LEKE
Specificity This antibody detects a single 52-55 kDa Fox3p protein in PC-Fox3p samples. It also reacts to a smaller (25-29 kD) protein in many cells lines, the identitiy of this protein is not known.
Purification Affinty chromatography
Alternative Name FOXP3
Background Forkhead box protein P3 (Fox3p) is a probable transcription factor that plays a critical role in the control of immune response. Mice deficient in FOX3P (Scurfy mice) exhibited over proliferation of CD4+CD8- T lymphocytes, multi-organ infilteration and elevation of numerous cytokines. The Fox3P deficiency is lethal in hemizygous mice similar to CTLA4 or TGFbeta deficient mice, the pathology observed is in scurfy mice is similar to animals that lack expression of CTLA4 or TGF beta that result from inability to properly regulate CD4+CD8- T cell activity (1). strong correlation between expression of forkhead box P3 and ex vivo expression of CTLA-4 in Treg cells was observed. The protein encoded by scurfin gene, designated as FOX3P is a new member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved in humans and is essential for normal immune homeostasis. FoxP3 is a necessary transcription factor for the development and function of CD4+ regulatory T-cells (Tregs) and it inhibits HIV infection by inhibiting NFAT activity and NFAT proteins contribute to HIV-1 transcription (2). Mutations in FOX3P gene (homolog of mouse scurfy gene) resulted in IPEX (X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome) disorder characterized by immune disorder characterized by immune dys-regulation, insulin dependent diabetes mellitus, infection, secretory diarrhea, thrombocytopenia, anemia and aczema. It is usually lethal in infancy. The CD4+CD25+ exert their suppressor function by regulating miR-142-3p via Adenylyl cyclase 9 (AC9) that inhibits AC9 production in Tregs. The FOX3P down-regulates miR-142-3p to keep the AC9-cAMP pathway active in Cd4+CD25+ Treg (3). The forkhead box p3 (Foxp3) positive Tregs have functionally immunosupressive properties that prevents effector cells from acting against self in autoimmune diseases/tumors are are highly relevant in cancer progression (4). Fox3P is a soluble nuclear protein containing 431 amino acids (MW 52kDa) protein contains 1 C2H2-type zing finger and one forkhead DNA-binding domain. Fox3P is expressed in several splice variant forms. Epigenetic regulation of Fox3P locus contributes to its role as a lineage-specification factor for development of Tregs. The Fox3PP gene is located on chromosome Xp11.23-Xq13.3. Forkhead (FH), also known as a ’winged helix’, is named for the Drosophila fork head protein, a transcription factor which promotes terminal rather than segmental development. Disruption in Fox3pp/winged helix/scurfy protein led lethal lymphoproliferative disorder in scurfin mouse. The scurfin has several FH (forkhead DNA binding domains near C-terminal end of the protein. At least 2 variant isoforms are expressed, a longer iso-form a and a short iso-form b.
Alternate names: Forkhead box protein P3, IPEX, JM2, Scurfin
Gene ID 20371
NCBI Accession NP_473380
UniProt Q99JB6
Research Area Immunology, Adaptive Immunity, Transcription Factors
Application Notes Western blot: 1: 500. IMM and i. p pull-down assays: > 1: 200 (2-5 µl/250 µg protein extracts. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.65 - 1.25 mg/mL
Buffer Stabilization buffer with 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Expiry Date 12 months
Background publications Brunkow, Jeffery, Hjerrild et al.: "Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse." in: Nature genetics, Vol. 27, Issue 1, pp. 68-73, 2001 (PubMed).

Selliah, Zhang, White et al.: "FOXP3 inhibits HIV-1 infection of CD4 T-cells via inhibition of LTR transcriptional activity." in: Virology, Vol. 381, Issue 2, pp. 161-7, 2008 (PubMed).

Huang, Zhao, Lei et al.: "miR-142-3p restricts cAMP production in CD4+CD25- T cells and CD4+CD25+ TREG cells by targeting AC9 mRNA." in: EMBO reports, Vol. 10, Issue 2, pp. 180-5, 2009 (PubMed).

Jang: "Prevalence of Foxp3 positive T regulatory cells is increased during progression of cutaneous squamous tumors." in: Yonsei medical journal, Vol. 49, Issue 6, pp. 942-8, 2008 (PubMed).

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