MutS Homolog 4 (E. Coli) (MSH4) antibody

Antigen: MutS Homolog 4 (E. Coli) (MSH4)

Synonyms: mMsh4, AV144863, 4930485C04Rik, MSH4

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN493135

Additional Information

Characteristics: Synonyms: MutS protein homolog 4

Alternative name: MSH4

Swiss-Prot: Q6DTM5

Immunogen: Synthetic peptide corresponding to aa 774-792 of MSH4

Description: Six strong homologs of the bacterial MutS DNA mismatch repair (MMR) gene have beenidentified in the yeast Saccharomyces cerevisiae. With the exception of the MSH1 gene, theinvolvement of each homolog in DNA repair and recombination during meiosis has beendetermined previously. Five of the homologs have been demonstrated to act in meioticDNA repair (MSH2, MSH3, MSH6 and MSH4) and/or meiotic recombination (MSH4 andMSH5). Several strong homologs of bacterial MutS DNA mismatch repair (MMR) genes havebeen identified (MSH1-MSH6), with the exception of the MSH1 gene, all others havesignificant role in recombination and DNA repair during meiosis. MSH4, a meiosis-specific member of the MutS-homolog family of genes, is required fornormal levels of recombination and fertility in budding yeast, mouse, and Caenorhabditiselegans. The arabipdosis specific homolog of MSH4 (AtMSH4) in reproduction wascharacterized in floral tissues (1). The expression of MSH4 in A thaliana is limited to earlymeiotic phase I preceding the synapsis of homologous chromosomes. The insertion ofAT-DNA mutant (ATmsh4) exhibited normal vegetative growth but significant reduction infertility consistent with meiotic defect which was further confirmed by cytological stainingof meiosis. Similar reduction in meiosis was observed with RNAi dependent downregulation of MSH4 which is the result of delayed or incomplete prophase I chromosomesysnapsis and metaphase chiasma frequency is greatly reduced compared to wild typeleading to univalence and nondisjunction in budding yeast (1). MSH4 protein has several function domains in cluding MutS domain III which is found inMutS family of DNA mismatch repair protein and is found associated with pfam00488,pfam05188, pfam01624 and pfam05190, pfam05192. The MutS1 homolog in eukaryotes. The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpairedbases embedded in duplex DNA and activating endo- and exonucleases to remove themismatch. A thaliana MSH4 protein is a 792 amino acid (98-100kDa) protein expressed dinnuclear compartment.

Specificity: This antibody labels MSH4 (C-term) at 98-100 kDa in Western blot. Species Reactivity: Tested: Arabidopsis

Application Details

Application Notes: Western blot: 1: 500. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.95 - 1.04 mg/ml (lot specific)

Purification: Aff - Purified

Buffer: Stabilization buffer

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only