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ADCY4 antibody (Adenylate Cyclase 4) (aminated)

Details for Product anti-ADCY4 Antibody No. ABIN493195, Supplier: Log in to see
Antigen
  • ADCY4
  • mKIAA4004
  • AC4
Alternatives
anti-Human ADCY4 antibody for Western Blotting
Epitope
aminated
16
15
12
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
47
27
24
Host
Rabbit
50
Clonality
Polyclonal
Conjugate
This ADCY4 antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
34
28
14
11
4
3
2
2
1
1
1
Supplier
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Immunogen Synthetic peptide (amindated) unique to PAC-4 protein
Specificity This antibody detects Adenylate cyclase type 4. It labels a 127-130 kDa protein in Western blot
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography
Alternative Name Adenylate Cyclase Type 4 (ADCY4 Antibody Abstract)
Background The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier (10). Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCrs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that in turn can regulate various metabolic pathways, gene expression, and affect memory functions etc., (3, 4). The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi hetero-trimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of of GDP to GTP that is bound to G protein. The GTP binding reduces the affinity of Gs(a) to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase (5). The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes. Adenylate cyclase Type 4 is located on chromosome type 11.Synonyms: ADCY4, ATP pyrophosphate-lyase 4, Adenylate cyclase type IV, Adenylyl cyclase 4
Gene ID 196883
NCBI Accession NP_640340
UniProt Q8NFM4
Pathways EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.75 - 1.2 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Taussig, Tang, Hepler et al.: "Distinct patterns of bidirectional regulation of mammalian adenylyl cyclases." in: The Journal of biological chemistry, Vol. 269, Issue 8, pp. 6093-100, 1994 (PubMed).

Gilman: "G proteins: transducers of receptor-generated signals." in: Annual review of biochemistry, Vol. 56, pp. 615-49, 1987 (PubMed).

Bourne, Sanders, McCormick: "The GTPase superfamily: a conserved switch for diverse cell functions." in: Nature, Vol. 348, Issue 6297, pp. 125-32, 1990 (PubMed).