Adenylate Cyclase 4 (ADCY4) (aminated) antibody

Details for Product No. ABIN493195
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Antigen
Synonyms ADCY4, mKIAA4004, AC4
Epitope
aminated
(11), (8), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(38), (25), (24), (12), (12), (12), (1)
Host
Rabbit
(43)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(31), (22), (10), (7), (4), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN493195
346.50 $
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Immunogen Synthetic peptide (amindated) unique to PAC-4 protein
Specificity This antibody detects Adenylate cyclase type 4. It labels a 127-130 kDa protein in Western blot
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography
Alternative Name Adenylate Cyclase Type 4
Background The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier (10). Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCrs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that in turn can regulate various metabolic pathways, gene expression, and affect memory functions etc., (3, 4). The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi hetero-trimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of of GDP to GTP that is bound to G protein. The GTP binding reduces the affinity of Gs(a) to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase (5). The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes. Adenylate cyclase Type 4 is located on chromosome type 11.Synonyms: ADCY4, ATP pyrophosphate-lyase 4, Adenylate cyclase type IV, Adenylyl cyclase 4
Gene ID 196883
NCBI Accession NP_640340
UniProt Q8NFM4
Application Notes Western blot 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.75 - 1.2 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Bourne, Sanders, McCormick: "The GTPase superfamily: a conserved switch for diverse cell functions." in: Nature, Vol. 348, Issue 6297, pp. 125-32, 1990 (PubMed).

Gilman: "G proteins: transducers of receptor-generated signals." in: Annual review of biochemistry, Vol. 56, pp. 615-49, 1987 (PubMed).

Taussig, Tang, Hepler et al.: "Distinct patterns of bidirectional regulation of mammalian adenylyl cyclases." in: The Journal of biological chemistry, Vol. 269, Issue 8, pp. 6093-100, 1994 (PubMed).

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