phosphodiesterase 4A, CAMP-Specific (PDE4A) (cyclized), (C-Term) antibody

Details for Product No. ABIN493249
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Antigen
Synonyms pde4, dpde2, pde46, PDE4A, Pde4a, D9Ertd60e, Dpde2, DPDE2, PDE4, PDE46, PHOSA
Epitope
cyclized, C-Term
(11), (8), (7), (7), (3), (2), (1), (1), (1)
Reactivity
Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus)
(54), (20), (19), (13), (12), (12), (12), (2), (1)
Host
Rabbit
(48), (19)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(36), (22), (18), (14), (11), (10), (10), (7), (5), (3), (2)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN493249
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Immunogen C-terminal synthetic cyclic peptide (common to all PDE4A proteins)
Specificity This antibody detects all known variants of PDE4A, including PDE4A1, PDE4A5, PDE4A8, PDE4Ax and a 76 kDa testis specific PDE4A protein.
Purification Affinity chromatography
Alternative Name PDE4A
Background Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). PDE4 enzymes are also important molecular targets for variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprised of 4 genes (PDE4A, B, C and D), each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants (2). Rolipram, an antidepressant drug, inhibits all the members of PDE4 family in mM concentration range. In rodents, inhibition of PDE4 enzymes attenuated short- and long- term memory impairment produced by scopolamine and MK801 administration (3). Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases (4). Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling (5). In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7), while in testis PDE4A variants are expressed exclusively in primary and secondary germ cells and are believed to be responsible for normal spermatogenesis (8).
Alternate names: 2EL, 5'-cyclic phosphodiesterase 4A, DPDE2, PDE4, PDE46, PDE4A1, PDE4A10, PDE4A11, PDE4A4, PDE4A4B, PDE4A6, PDE4A7, PDE4A8, PDE4A8A, Phosphodiesterase 4A, Phosphodiesterase-4A cAMP-specific, RD1, cAMP-specific 3'
Gene ID 5141
NCBI Accession NP_001104777
UniProt P27815
Research Area Signaling
Application Notes ELISA. Immunocytochemistry: < 1: 250. Immunoprecipitation: < 1: 250. Western blot: < 1: 1000.
Restrictions For Research Use only
Format Liquid
Concentration 0.65 - 1.0 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Expiry Date 12 months
Background publications Beavo: "Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms." in: Physiological reviews, Vol. 75, Issue 4, pp. 725-48, 1995 (PubMed).

Yarwood, Steele, Scotland et al.: "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14909-17, 1999 (PubMed).

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