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phosphodiesterase 4A, CAMP-Specific (PDE4A) (phosphospecific) antibody

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Synonyms pde4, dpde2, pde46, PDE4A, Pde4a, D9Ertd60e, Dpde2, DPDE2, PDE4, PDE46, PHOSA
(22), (15), (8), (5), (3), (2), (2), (1), (1), (1), (1)
Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus)
(77), (22), (19), (4), (3), (2), (2)
(52), (26)
(5), (3), (2), (2), (2), (2)
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(72), (32), (27), (23), (21), (19), (18), (18), (4), (2), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide (common to all long-form PDE4A proteins)
Specificity This antibody detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax only when they are phosphorylated by protein kinase A (PKA) pathway. It does not cross react with PDE4B, PDE4D or any other member of the PDE family.
Cross-Reactivity (Details) Species reactivity (tested):Human, monkey, mouse, rat, chicken
Purification Affinity chromatography
Alternative Name PDE4A (PDE4A Antibody Abstract)
Background Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90 % of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7). In testis PDE4A variants are expressed exclusively in primary and secondary germ cells and are believed to be responsible for normal spermatogenesis (8). PDE4 enzymes are also important molecular targets for variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprised of 4 genes (PDE4A, B, C and D), each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants (2). Rolipram, an antidepressant drug, inhibits all the members of PDE4 family in mM concentration range. In rodents, inhibition of PDE4 enzymes attenuated short- and long-term memory impairment produced by scopolamine and MK801 administration (3). Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases (4). Two conserved phosphorylation motifs have been identified in PDE4A subtype variants (i) Phosphorylation at PKA site and (ii) an ERK kinase site. Phosphorylation at PKA site resulted in significant increase I enzymatic activity of PDE4A variants. Phosphorylation state, protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling (5).Synonyms: 2EL, 5'-cyclic phosphodiesterase 4A, DPDE2, PDE4, PDE46, PDE4A1, PDE4A10, PDE4A11, PDE4A4, PDE4A4B, PDE4A6, PDE4A7, PDE4A8, PDE4A8A, Phosphodiesterase 4A, Phosphodiesterase-4A cAMP-specific, RD1, cAMP-specific 3'
Gene ID 5141
NCBI Accession NP_001104777
UniProt P27815
Research Area Signaling
Application Notes ELISA. Flow cytometry. Immunoflourescence. Immunoprecipitation. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 - 0.6 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Farooqui, Al-Bagdadi, Houslay et al.: "Surgically induced cryptorchidism-related degenerative changes in spermatogonia are associated with loss of cyclic adenosine monophosphate-dependent phosphodiesterases type 4 in abdominal testes of rats." in: Biology of reproduction, Vol. 64, Issue 6, pp. 1583-9, 2001 (PubMed).

Yarwood, Steele, Scotland et al.: "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14909-17, 1999 (PubMed).

Beavo: "Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms." in: Physiological reviews, Vol. 75, Issue 4, pp. 725-48, 1995 (PubMed).

Farooqui, Brock, Hamdi et al.: "Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum." in: Journal of neurochemistry, Vol. 57, Issue 4, pp. 1363-9, 1991 (PubMed).

Catalog No. ABIN493251
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