phosphodiesterase 4D, CAMP-Specific (PDE4D) (phosphospecific) antibody

Details for Product No. ABIN493281
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Antigen
Synonyms 9630011N22Rik, Dpde3, ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1
Epitope
phosphospecific
(12), (11), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus)
(58), (43), (42), (12), (12), (12), (1), (1)
Host
Rabbit
(66), (10), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(60), (18), (14), (14), (12), (10), (6), (5), (3), (2), (2)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN493281
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Immunogen Synthetic peptide common to all long-form PDE4D proteins
Specificity This antibody detects all known long-from variants of PDE4D, including PDE4D2, PDE4D3, PDE4D4, PDE4D5 when they are phosphorylated by protein kinase A (PKA) pathway. The epitope is on PKA site and will not label PDE4D proteins phosphorylated at ERK kinase site. Thus making this antibody an ideal tool for studying PKAdependent activation of PDEs. The antibody does not cross react with PDE4B, PDE4A, PDE4C or any other member of the PDE family.
Cross-Reactivity (Details) Species reactivity (tested):Human, monkey, chicken, mouse, rat
Purification Affinity chromatography
Alternative Name PDE4D
Background Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90 % of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7). ERK mitogen activated protein kinase and cAMP pathways are important regulators for cross talk between. In testis PDE4A variants are expressed exclusively in primary and secondary germ cells and are believed to be responsible for normal spermatogenesis (8). PDE4 enzymes are also important molecular targets for variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprised of 4 genes (PDE4A, B, C and D), each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants (2). Rolipram, an antidepressant drug, inhibits all the members of PDE4 family in mM concentration range. In rodents, inhibition of PDE4 enzymes attenuated short- and long-term memory impairment produced by scopolamine and MK801 administration (3). Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases (4). Two conserved phosphorylation motifs have been identified in PDE4B, C and D, the PDE4A has a single phosphorylation site (i) Phosphorylation at PKA site and (ii) an ERK kinase site. Phosphorylation at PKA site resulted in significant increase in enzymatic activity of PDE4D variants. Phosphorylation state, protein-protein interactions and cellular trafficking of PDE4D enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling (5).Synonyms: 5'-cyclic phosphodiesterase 4D, DPDE3, EC=3.1.4.17, PDE43, PDE4D, cAMP-specific 3'
Gene ID 5144
NCBI Accession NP_001098101
UniProt Q08499
Research Area Signaling, Enzymes, Metabolism
Application Notes Flow cytometry. Immunflourescence.
Restrictions For Research Use only
Concentration 0.5 - 0.75 mg/mL
Buffer Stabillization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Beavo: "Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms." in: Physiological reviews, Vol. 75, Issue 4, pp. 725-48, 1995 (PubMed).

Yarwood, Steele, Scotland et al.: "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14909-17, 1999 (PubMed).

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