phosphodiesterase 4D, CAMP-Specific (PDE4D) (pSer579) antibody

Details for Product No. ABIN493282
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Synonyms 9630011N22Rik, Dpde3, ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1
(12), (11), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus)
(58), (43), (42), (12), (12), (12), (1), (1)
(66), (10), (1)
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(60), (18), (15), (14), (12), (10), (7), (5), (3), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN493282
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Immunogen Synthetic peptide common in long-form PDE4D proteins
Sequence STI PQS(p) PSP APD
Specificity This antibody detects all known long-from variants of PDE4D, including PDE4D2, PDE4D3, PDE4D4, PDE4D5 when they are phosphorylated by ERK pathway.
Cross-Reactivity (Details) Species reactivity (tested):Human, monkey, chicken, mouse, rat
Purification Affinity chromatography
Alternative Name PDE4D
Background Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90 % of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7). ERK mitogen activated protein kinase and cAMP pathways are important regulators for cross talk between protein kinases and cAMP at distinct points in the cell (2). The phosphodiesterase type 4b, C and D sbtypes, but not the PDE4A subtype, have a consensus ERK site phosphorylation site. This site in PDE4D3 lies at serine at position 579 (3-5). The ERK kinase phosphorylation site lies in the consensus motif Pro-Xaa-Ser-Pro. In vivo, a KIM motif (Val-Xaa-Xaa-Lys-Lys-Xaa6-Leu-Leu-Leu-Xaa12-Phospho-serine) serves as a docking site for ERK/JNK and is located upstream to the target phosphorylation site. The specificty for ERK is confered over JNK by the presence of FQF motif down stream from the phosphorylation site (6). PDE4B, C and D all have conserved KIM and FQF sites that provide serine as substrate for ERK phosphorylation. The crystal structure and the three dimentional modeling of the PDE4 D/B suggest that both KIM and FQF domain are located on a single microdoamin, the KIM being located on exposed hair pin loop and the FQF on an exposed a helix (6). The location of these doamins excelently displaced for ERK internations. The phosphorylation of PDE4B, C, D long-form variants by ERK kinases led to a significant inhibition (approximately 80 %) in PDEase activity (3-5). ERK phosphorylation of PDE4 long-forms cause an increse in cAMP as a resultof inhibition in PDEase activity. The rise in cAMP levels consequentially activated the cAMP-dependent PKA that phosphorylates the same protiens at PKA site (serine 53 in PDE4D3) that led to a significant increase in PDEase activity (7). Taken together these effects will generate a traanssient increase in cAMP lervels in a cytosolic sub-compartment.Synonyms: 5'-cyclic phosphodiesterase 4D, DPDE3, EC=, PDE43, PDE4D, cAMP-specific 3'
Gene ID 5144
NCBI Accession NP_001098101
UniProt Q08499
Research Area Signaling, Enzymes, Metabolism
Application Notes Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 - 0.75 mg/mL
Buffer Stabilization buffer with 0.02 % thimerosal or merthiolate
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Beavo: "Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms." in: Physiological reviews, Vol. 75, Issue 4, pp. 725-48, 1995 (PubMed).

Behar, Bisello, Bitan et al.: "Photoaffinity cross-linking identifies differences in the interactions of an agonist and an antagonist with the parathyroid hormone/parathyroid hormone-related protein receptor." in: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 9-17, 2000 (PubMed).

Burke, Gambliel, Olson et al.: "Prevention by dexrazoxane of down-regulation of ryanodine receptor gene expression in anthracycline cardiomyopathy in the rat." in: British journal of pharmacology, Vol. 131, Issue 1, pp. 1-4, 2000 (PubMed).

Houslay, Baillie: "The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways." in: Biochemical Society transactions, Vol. 31, Issue Pt 6, pp. 1186-90, 2003 (PubMed).

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