phosphodiesterase 7B (PDE7B) antibody

Antigen: phosphodiesterase 7B (PDE7B)

Synonyms: MGC88256, bA472E5.1, PDE7B, MGC151559, ba472e5.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN493314

Additional Information

Characteristics: Synonyms: Phosphodiesterase 7B, MGC88256, cAMP-specific 3', 5'-cyclic phosphodiesterase 7B,rolipram-insensitive phosphodiesterase type 7

Alternative name: PDE7B

Gene ID: 27115

Swiss-Prot: Q9NP56

Immunogen: Synthetic cyclic peptide (common to all PDE7B variants)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] andcyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells andact as second messenger transducers to effect the intracellular actions of a variety ofG-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play importantrole in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed andcompartmentalized by a family of enzymes called phosphodieterases. One of the manyphosphodiesterases that compartmentalized and hydrolyze cAMP in T-Lymphocytes arephosphodiesterase type 7. The cAMP-specific phosphodiesterase type-7 (PDE7) family iscomprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated byRNA splicing and use of alternate initiation sites (1). PDE7B has three splice variants(PDE7B1 = 446 amino acid, PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences. Like other PDEs, human PDE7B gene products have an apparent molecular weight of 53-55kDa on reduced SDS-PAGE (2). As in PDE7A, the PDE7B has a significant conserved regionof about 270 amino acids common to all PDEs at the carboxy terminal apparently serves asthe catalytic domain. The amino-terminal region of this protein is divergent andpresumably accounts for the distinctive and regulatory properties unique to the individualPDE families. PDE7B protein showed significant homology to other cAMP-dependent PDEs(23%) with in the catalytic domain. PDE7B is expressed in various tissues includingskeletal muscle, heart, lung and testis (2).

Specificity: This antibody detects a 50-55 kDa PDE7B protein in various rat tissues. Species Reactivity: Tested: Human, mouse, rat

Application Details

Application Notes: Western blot: > 1: 500. IMM and i. p pull-down assays: > 1: 250.

Concentration: 0.5 - 0.6 mg/ml (lot specific)

Purification: Aff - Purified

Buffer: Antibody stabilization buffer

Storage: Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Enzymes, Metabolism

Restrictions: For Research Use only