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Antibodies
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anti-phosphodiesterase 9A (PDE9A) antibody
phosphodiesterase 9A (PDE9A) antibody
| Antigen |
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| Synonyms |
HSPDE9A2, PDE9A1, PDE9A, MGC116558, Pde9a, LOC799942 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN493326 |
| Quantity |
0.1 mg (0.85 - 1.25 mg/ml (lot specific)) (Variants) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Phosphodiesterase 9A, Phosphodiesterase PDE9A21, HSPDE9A2, High affinitycGMP-specific 3', 5'-cyclic phosphodiesterase 9A
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Alternative name
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PDE9A
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Gene ID
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5152
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Swiss-Prot
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O76083
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Immunogen
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Synthetic cyclic peptide (common to all PDE9A variants)
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Cross-Reactivity
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Human, Mouse (Murine), Rat (Rattus)
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Description
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Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants(PDE4D1, D2, D3, D4, D6, D7, D8 and D9, (1, 2). One or more PDE4D subtype-variants areubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants areexpressed in varying ratios and their activity is regulated in tandem with GPCRs stimulationand selective phosphorylation by PKA and other kinases. Peripheral tissues also exhibitdifferential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to anincrease in cAMP (3). Short-term regulation of PDE4D variants involved PKA, MAP kinasesand Erk2 phosphorylation that results in rapid change in their enzymatic activities (2, 4). Other regulatory mechanism involved protein-protein interactions with cytoskeletalscaffolding proteins (5). Anti-PDE4D antibodies are ideal tools for studying regulation,expression, phosphorylation, and protein-protein interactions of PDE4D variantsemploying pull-down immunoprecipitation protocol.
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Specificity
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This antibody detects Phosphodiesterase 9A. Species Reactivity: Tested: Human, mouse, rat
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Application Notes
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Western blot: > 1: 500. IMM and i. p pull-down assays: > 1: 250. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.85 - 1.25 mg/ml (lot specific)
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Purification
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Aff - Purified
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Buffer
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Stabilization buffer
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Storage
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Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Signaling, Enzymes, Metabolism
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Restrictions
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For Research Use only
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Alternatives for antigen "phosphodiesterase 9A (PDE9A)", type "Antibodies"
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Hosts
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Rabbit (8), Mouse (5)
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Reactivities
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Human (10), Mouse (Murine) (3), Rat (Rattus) (3)
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Applications
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Western Blotting (WB) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (Detection) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunoprecipitation (IP) (3), ELISA (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Immunohistochemistry (IHC) (2)
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