Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Itoh, Ding, Bains, Wang, Takeishi, Jalili, King, Walsh, Yan, Abe: "Role of p90 ribosomal S6 kinase (p90RSK) in reactive oxygen species and protein kinase C beta (PKC-beta)-mediated cardiac troponin I phosphorylation." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 24135-42, (2005) (PubMed).
Silverman, Frödin, Gammeltoft, Maller: "Activation of p90 Rsk1 is sufficient for differentiation of PC12 cells." in: Molecular and cellular biology, Vol. 24, Issue 24, pp. 10573-83, (2004) (PubMed).
Eisenmann, VanBrocklin, Staffend, Kitchen, Koo: "Mitogen-activated protein kinase pathway-dependent tumor-specific survival signaling in melanoma cells through inactivation of the proapoptotic protein bad." in: Cancer research, Vol. 63, Issue 23, pp. 8330-7, (2003) (PubMed).
Target
RPS6KA1
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))