HNRNPF antibody (N-Term)

Catalog No. ABIN497944

Product Details anti-HNRNPF Antibody

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Binding Specificity: AA 1-164, N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes hnRNP-F/HNRNPF (1-174).
Cellular Localization: Nucleus/nucleoplasm

Cross-Reactivity (Details): Species reactivity (expected):Mouse (98 %), Rat (83 %), Bovine (97 %).
Species reactivity (tested):Human.

Purification: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of hnRNP-F

Application Details

Application Notes: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100-1/250. Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.72 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 20 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Alternative Name: HnRNP-F / HNRNPF (HNRNPF Products)

Synonyms: HNRPF antibody, OK/SW-cl.23 antibody, mcs94-1 antibody, HNRNPF antibody, Hnrpf antibody, 4833420I20Rik antibody, AA407306 antibody, QtsA-10715 antibody, hnRNP F antibody, heterogeneous nuclear ribonucleoprotein F antibody, HNRNPF antibody, Hnrnpf antibody

Background: HnRNP-F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.Synonyms: HNRPF, Heterogeneous nuclear ribonucleoprotein F, Nucleolin-like protein mcs94-1

Gene ID: 3185, 9606

UniProt: P52597