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ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (N-Term)

Details for Product anti-ATG5 Antibody No. ABIN497966, Supplier: Log in to see
Antigen
  • ATG5
  • CG1643
  • DmAtg5
  • Dmel\\CG1643
  • atg5
  • apg5l
  • zgc:100934
  • ATATG5
  • AUTOPHAGY 5
  • MKP11.20
  • MKP11_20
  • APG5
  • APG5-LIKE
  • APG5L
  • ASP
  • hAPG5
  • 2010107M05Rik
  • 3110067M24Rik
  • AW319544
  • Apg5l
  • Atg5l
  • C88337
  • Paddy
  • Autophagy-specific gene 5
  • autophagy related 5
  • APG5 autophagy 5-like
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • autophagy protein 5
  • similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12
  • Atg5
  • atg5
  • LOC100227318
  • LOC100342807
  • ATG5
  • APG5
Alternatives
anti-Human ATG5 antibody for Western Blotting
Epitope
N-Term
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32
22
15
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11
8
8
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5
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3
3
2
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2
1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
284
125
92
37
34
23
19
12
12
10
6
5
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4
4
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3
1
1
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Host
Rabbit
219
49
14
12
2
Clonality
Polyclonal
Conjugate
This ATG5 antibody is un-conjugated
12
12
12
10
10
10
2
2
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Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
232
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73
50
39
13
12
6
4
2
2
2
1
1
1
1
Options
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Immunogen A 16 amino acid peptide from near the amino terminus of Human ATG5
Isotype IgG
Specificity Recognizes APG5L/ATG5.
Purification Affinity Chromatography purified via peptide column.
Alternative Name APG5L / ATG5 (ATG5 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5
Gene ID 9474
NCBI Accession NP_004840
UniProt Q9H1Y0
Pathways Activation of Innate immune Response
Application Notes ELISA. Western Blot: ATG5 antibody can be used for the detection of ATG5 at 1-2 μg/mL. Positive Control. Rat Spleen Tissue Lysate. Immunohistochemistry. Positive Control. Human Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (N-Term) (ABIN497966) Immunohistochemistry: ATG5 antibody staining of Rat Spleen tissue ly...
Western Blotting (WB) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (N-Term) (ABIN497966) Western blot. ATG5 antibody staining of Rat Spleen tissue lysate at ...
Background publications Hanada, Noda, Satomi, Ichimura, Fujioka, Takao, Inagaki, Ohsumi: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 52, pp. 37298-302, 2007 (PubMed).

Jounai, Takeshita, Kobiyama, Sawano, Miyawaki, Xin, Ishii, Kawai, Akira, Suzuki, Okuda: "The Atg5 Atg12 conjugate associates with innate antiviral immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 35, pp. 14050-5, 2007 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).