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ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (N-Term)

Details for Product anti-ATG5 Antibody No. ABIN497967, Supplier: Log in to see
Antigen
  • 2010107M05Rik
  • 3110067M24Rik
  • APG5
  • APG5-LIKE
  • Apg5l
  • APG5L
  • apg5l
  • ASP
  • ATATG5
  • atg5
  • ATG5
  • Atg5l
  • AUTOPHAGY 5
  • AW319544
  • C88337
  • CG1643
  • DmAtg5
  • Dmel\\CG1643
  • hAPG5
  • MKP11.20
  • MKP11_20
  • Paddy
  • zgc:100934
Epitope
N-Term
60
31
14
14
14
12
11
8
8
7
7
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
271
103
80
28
25
22
19
12
6
5
3
3
2
2
1
1
1
1
Host
Chicken
211
47
14
11
2
Clonality
Polyclonal
Conjugate
This ATG5 antibody is un-conjugated
12
12
12
10
10
10
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
228
121
118
38
36
14
12
12
6
4
2
2
2
1
1
1
1
Supplier
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Immunogen Chicken polyclonal ATG5 antibody was raised against a 16 amino acid peptide from near the amino terminus of Human ATG5.
Isotype Ig
Specificity Recognizes APG5L/ATG5.
Purification Affinity Chromatography purified via peptide column.
Alternative Name APG5L / ATG5 (ATG5 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5
Gene ID 9474
NCBI Accession NP_004840
UniProt Q9H1Y0
Application Notes ELISA. Western Blot: ATG5 antibody can be used for the detection of ATG5 at 1-2 μg/mL. Positive Control. Mouse Spleen Tissue Lysate. Immunohistochemistry. Positive Control. Human Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (N-Term) (ABIN497967) Immunohistochemistry: ATG5 antibody staining of Human Spleen tissue ...
Western Blotting (WB) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (N-Term) (ABIN497967) Western blot. ATG5 antibody staining of Mouse Spleen tissue lysate a...
Background publications Hanada, Noda, Satomi, Ichimura, Fujioka, Takao, Inagaki, Ohsumi: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 52, pp. 37298-302, 2007 (PubMed).

Jounai, Takeshita, Kobiyama, Sawano, Miyawaki, Xin, Ishii, Kawai, Akira, Suzuki, Okuda: "The Atg5 Atg12 conjugate associates with innate antiviral immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 35, pp. 14050-5, 2007 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).