ATG7 antibody (N-Term)

Catalog No. ABIN497975

Product Details anti-ATG7 Antibody

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: Recognizes APG7L/ATG7 (N-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Immunoaffinity Chromatography

Immunogen: APG7 antibody was raised against a 17 amino acid peptide from near the amino terminus of Human APG7.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: APG7 antibody can be used for the detection of APG7 at 1-2 μg/mL. Positive Control: L1210 Cell Lysate. Immunocytochemistry. Positive Control: L1210 Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: APG7L / ATG7 (ATG7 Products)

Synonyms: APG7-LIKE antibody, APG7L antibody, GSA7 antibody, D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, AO090001000744 antibody, atg7 antibody, 1810013K23Rik antibody, Agp7 antibody, Apg7l antibody, Atg7l antibody, ATAPG7 antibody, ATATG7 antibody, ATG7 antibody, AUTOPHAGY 7 antibody, AUTOPHAGY-RELATED 7 antibody, K15I22.10 antibody, K15I22_10 antibody, autophagy related 7 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, autophagy-related protein 7 antibody, Autophagy-related protein 7 antibody, Apg7p antibody, ThiF family protein antibody, ubiquitin-like conjugating enzyme Atg7 antibody, ATG7 antibody, UBA7 antibody, Uba7 antibody, ANI_1_3368024 antibody, AOR_1_1338164 antibody, PTRG_02236 antibody, PAAG_00287 antibody, PITG_12537 antibody, atg7 antibody, Atg7 antibody, APG7 antibody

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme, this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).Synonyms: APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

Gene ID: 10533

NCBI Accession: NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy