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LAMP2 antibody (Lysosomal-Associated Membrane Protein 2) (C-Term)

Details for Product anti-LAMP2 Antibody No. ABIN497979, Supplier: Log in to see
Antigen
  • cd107b
  • lamp-2
  • lampb
  • LAMP2
  • lamp2
  • DKFZp459J126
  • LAMP-2
  • LGP-B
  • CD107b
  • Lamp-2
  • Lamp-2a
  • Lamp-2b
  • Lamp-2c
  • Mac3
  • LAMPB
  • LGP110
  • lysosomal-associated membrane protein 2
  • Lysosome-associated membrane glycoprotein 2
  • lysosomal membrane glycoprotein B
  • LAMP2
  • lamp2
  • LOC100218047
  • LOC100222683
  • Lgpb
  • Lamp2
Epitope
C-Term
20
17
16
14
12
12
12
10
7
5
5
5
4
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
292
213
46
41
19
9
3
2
Host
Rabbit
162
147
118
2
Clonality
Polyclonal
Conjugate
This LAMP2 antibody is un-conjugated
53
39
29
23
11
10
7
6
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
238
193
103
100
96
95
93
57
54
13
11
7
5
4
2
2
2
2
2
2
1
1
1
1
Options
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Immunogen LAMP2 antibody was raised against a 17 amino acid peptide from near the carboxy of Human LAMP-2 (NP_054701).
Isotype IgG
Specificity Recognizes CD107b/LAMP2 (C-term).
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Immunoaffinity Chromatography
Alternative Name CD107b / LAMP2 (LAMP2 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Gene ID 3920
NCBI Accession NP_054701
UniProt P13473
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 1-2 μg/mL. Despite its predicted size, LAMP-2 migrates at 115 kDa in SDS-PAGE. Positive Control: HepG2 Cell Lysate. Immunocytochemistry. Positive Control: HepG2 Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-LAMP2 antibody (Lysosomal-Associated Membrane Protein 2) (C-Term) (ABIN497979) AP20058PU-N CD107b/LAMP2 antibody staining of HepG2 cells by Immunocytochemistry at 1...
Western Blotting (WB) image for anti-LAMP2 antibody (Lysosomal-Associated Membrane Protein 2) (C-Term) (ABIN497979) AP20058PU-N CD107b/LAMP2 antibody staining of HepG2 cell lysate by Western Blotting a...
Background publications Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Tanaka, Guhde, Suter, Eskelinen, Hartmann, Lüllmann-Rauch, Janssen, Blanz, von Figura, Saftig: "Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice." in: Nature, Vol. 406, Issue 6798, pp. 902-6, 2000 (PubMed).

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Nishino, Fu, Tanji, Yamada, Shimojo, Koori, Mora, Riggs, Oh, Koga, Sue, Yamamoto, Murakami, Shanske, Byrne, Bonilla, Nonaka, DiMauro, Hirano: "Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease)." in: Nature, Vol. 406, Issue 6798, pp. 906-10, 2000 (PubMed).

Gough, Hatem, Fambrough: "The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c." in: DNA and cell biology, Vol. 14, Issue 10, pp. 863-7, 1995 (PubMed).

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Granger, Green, Gabel, Howe, Mellman, Helenius: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990 (PubMed).