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Details for Product No. ABIN497979

Lysosomal-Associated Membrane Protein 2 (LAMP2) (C-Term) antibody

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Antigen
Synonyms CD107b, LAMP-2, LAMPB, LGP110, Mac3, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, cd107b, lamp-2, lampb, LAMP2, lamp2, DKFZp459J126
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN497979
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen LAMP2 antibody was raised against a 17 amino acid peptide from near the carboxy of Human LAMP-2 (NP_054701).
Isotype IgG
Specificity Recognizes CD107b/LAMP2 (C-term). Species Reactivity: Tested: Human, Mouse.
Purification Immunoaffinity Chromatography
Alternative Name CD107b / LAMP2
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon’s disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).
Alternate names: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Gene ID 3920
NCBI Accession NP_054701
UniProt P13473
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 1–2 μg/ml. Despite its predicted size, LAMP-2 migrates at 115kDa in SDS-PAGE. Positive Control: HepG2 Cell Lysate. Immunocytochemistry. Positive Control: HepG2 Cell Lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Granger, Green, Gabel et al.: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990 (PubMed).

Gough, Hatem, Fambrough: "The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c." in: DNA and cell biology, Vol. 14, Issue 10, pp. 863-7, 1995 (PubMed).

Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Tanaka, Guhde, Suter et al.: "Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice." in: Nature, Vol. 406, Issue 6798, pp. 902-6, 2000 (PubMed).

Nishino, Fu, Tanji et al.: "Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease)." in: Nature, Vol. 406, Issue 6798, pp. 906-10, 2000 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Alternatives for antigen "Lysosomal-Associated Membrane Protein 2 (LAMP2)", type "Antibodies"
Hosts (80), (63), (50), (1)
Reactivities (112), (100), (24), (21), (13), (13), (7), (4)
Applications (102), (74), (38), (37), (32), (26), (22), (21), (20), (11), (10), (4), (2), (1)
Conjugates (28), (18), (12), (8), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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