Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (Middle Region) antibody

Details for Product No. ABIN497985
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Antigen
Synonyms DKFZp459H2130, 2210402P09Rik, AI844555, CAL, FIG, GOPC1, PIST, fe38f01, wu:fe38f01, zgc:56254, zgc:65853, dJ94G16.2, cal, gopc1, pist
Epitope
Middle Region
(30), (11), (7), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(89), (43), (32), (16), (15), (12), (12), (1), (1)
Host
Rabbit
(85), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(79), (49), (41), (14), (10), (5), (3), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN497985
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Immunogen 17 amino acid peptide from near the center of Human PIST (Genbank accession No. AAG00572).
Isotype IgG
Specificity Recognizes PIST (Intermediate Domain).
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Immunoaffinity Chromatography
Alternative Name GOPC / PIST
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1. (6)Synonyms: CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10
Gene ID 57120
NCBI Accession NP_001017408
UniProt Q9HD26
Application Notes ELISA. Western Blot: PIST antibody can be used for the detection ob PIST at 1-2 μg/mL. Positive Control: PC-3 Cell Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Colon Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.
Background publications Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Kametaka, Okano, Ohsumi et al.: "Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 273, Issue 35, pp. 22284-91, 1998 (PubMed).

Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Yue, Horton, Bravin et al.: "A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice." in: Neuron, Vol. 35, Issue 5, pp. 921-33, 2002 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

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