Did you know that you can buy products from over 140 different suppliers from us?

ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae) (ATG16L1) (N-Term) antibody

Details for Product No. ABIN497988
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms APG16L, ATG16A, ATG16L, IBD10, WDR30, 1500009K01Rik, Apg16l, Atg16l, Wdr30, atg16, atg16l
Epitope
N-Term
(14), (14), (12), (10), (10), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(130), (72), (60), (33), (14), (10), (6), (2), (1)
Host
Rabbit
(121), (9), (8), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(96), (46), (30), (25), (21), (10), (9), (4), (3), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN497988
401.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen ATG16 antibody was raised against a 18 amino acid peptide from near the amino terminus of Human ATG16 (Genbank accession No. NP_110430).
Isotype IgG
Specificity Recognizes ATG16 (N-term). Species Reactivity: Tested: Human, Mouse and Rat.
Purification Immunoaffinity Chromatography
Alternative Name APG16L / ATG16L1
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
Alternate names: APG16-like 1, Autophagy-related protein 16-1
Gene ID 55054
NCBI Accession NP_110430
UniProt Q676U5
Application Notes ELISA. Western blot: ATG16 antibody can be used for the detection of ATG16 at 0.5–1 μg/ml. Immunocytochemistry. Positive Control: HeLa Cell Lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Mizushima, Noda, Ohsumi: "Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway." in: The EMBO journal, Vol. 18, Issue 14, pp. 3888-96, 1999 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Mizushima, Kuma, Kobayashi et al.: "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1679-88, 2003 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN497988
401.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top