Neurotensin Receptor 2 (NTSR2) antibody

Antigen: Neurotensin Receptor 2 (NTSR2)

Synonyms: NT2R, NTRL, NTR2, NTSR2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN497996

Additional Information

Characteristics: Synonyms: NT-R-2, NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor

Alternative name: Neurotensin receptor type 2

Gene ID: 23620

Swiss-Prot: O95665

Immunogen: Synthetic peptide corresponding to 2nd cytoplasmic domain of Human NTSR2. Remarks: Mouse Identity: 93%Mouse Similarity: 93%Rat Identity: 93%Rat Similarity: 93%

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG  (Matching secondary antibodies)

Description: Neurotensin (NT) initiates an intracellular response by interacting with the Gprotein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor,levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and inperipheral tissues where NT receptors can contribute to hypotension, hyperglycemia,hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60cells express NTR1, which can couple to Gq, Gi/o or Gs. Alternative splicing of rat NTR2 cangenerate a 5-transmembrane domain variant isoform that is coexpressed with the fulllength NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglialcell line N11 induces MIP-2, MCP-1, IL-1β and TNFα in an ERK1/2 and Akt kinase-dependentmanner. NTR2, a Neurotensin Receptor, is primarily expressed in brain neurons and astrocytes. It isactivated by neurotensin and by levocabastine, a histamine H1 antagonist.

Specificity: Reacts with Neurotensin Receptor Type 2 (NTR2/NTSR2).

Application Details

Application Notes: Immunohistochemistry on Formalin Fixed Paraffin Embedded Tissues: 10-12 µg/ml(Requires pretreatment with Proteinase K). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS containing 0.09% Sodium Azide as preservative

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only