MLLT10 antibody (Internal Region)

Catalog No. ABIN498029

Product Details anti-MLLT10 Antibody

Target: MLLT10 (Protein AF-10 (MLLT10))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This MLLT10 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-RLEDTTARFT NAN

Specificity: This antibody is expected to recognize both reported isoforms (NP_004632.1, NP_001009569.1).

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Canine (Dog), Bovine.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004632.1, NP_001009569.1. Genename: MLLT10

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Experiments gave bands at approx 140 kDa and 60 kDa in RatTestis lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MLLT10

Target: MLLT10 (Protein AF-10 (MLLT10))

Alternative Name: MLLT10 / AF10 (MLLT10 Products)

Synonyms: AF10 antibody, Af10 antibody, B130021D15Rik antibody, D630001B22Rik antibody, mKIAA4140 antibody, MLLT10, histone lysine methyltransferase DOT1L cofactor antibody, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10 antibody, MLLT10 antibody, Mllt10 antibody

Background: AF10 is a putative transcription factor containing a leucine zipper motif. A t(10,11)(p12,q23) chromosomal translocation present in a proportion of acute myeloid leukaemias results in expression of an AF10 (carboxy terminus) MLL (amino terminus) fusion protein.Synonyms: myeloid/lymphoid or mixed-lineage leukemia translocated to 10

Gene ID: 8028

NCBI Accession: NP_001009569

UniProt: P55197