GABRG2 antibody (Internal Region)

Catalog No. ABIN498030

Product Details anti-GABRG2 Antibody

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Binding Specificity: AA 400-410, Internal Region

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This GABRG2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-NNATHLQERD E

Specificity: This antibody is expected to recognize all reported isoforms: NP_944494.1, NP_000807.2, NP_944493.2.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine.
Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_944494.1, NP_000807.2, NP_944493.2. Genename: GABRG2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in Mouse and Rat Brain lysates(calculated MW of 55.0 kDa according to Mouse NP_032099.1 and 54.0 kDa according to RatNP_899156.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GABRG2

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Alternative Name: GABRG2 (GABRG2 Products)

Synonyms: cae2 antibody, eca2 antibody, gabrg1 antibody, gefsp3 antibody, si:ch211-145n14.1 antibody, CAE2 antibody, ECA2 antibody, GEFSP3 antibody, GABAA-R antibody, Gabrg-2 antibody, gamma2 antibody, gamma-aminobutyric acid type A receptor gamma2 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, gamma 2 antibody, gamma-aminobutyric acid type A receptor gamma 2 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 antibody, GABRG2 antibody, gabrg2 antibody, Gabrg2 antibody

Background: GABA A receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA A. Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA A Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. The binding of GABA to GABA A receptors results in the increase transportation of chlorine ions into the neuron. This influx results in signal transmission repression. Studies have shown that if cell to cell signaling goes unchecked due to a lack of GABA, a system "overload" can resulting in blackouts, rapid body contractions, seizures and epilepsy.Synonyms: GABA A Receptor subunit gamma-2, GABRG-2, Gamma-aminobutyric acid receptor subunit gamma-2

Gene ID: 2566

NCBI Accession: NP_000807

UniProt: P18507