POU2F3 antibody (Internal Region)

Catalog No. ABIN498032

Product Details anti-POU2F3 Antibody

Target: POU2F3 (POU Class 2 Homeobox 3 (POU2F3))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This POU2F3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-EPHLEASQHL PVPKH

Specificity: Recognizes POU2F3.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_055167.2. Genename: POU2F3

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEK293 andHepG2 (calculated MW of 47.0 kDa according to NP_055167.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POU2F3

Target: POU2F3 (POU Class 2 Homeobox 3 (POU2F3))

Alternative Name: POU2F3 (POU2F3 Products)

Synonyms: POU2F3 antibody, zgc:136377 antibody, zgc:158534 antibody, Epoc-1 antibody, OCT-11 antibody, OCT11 antibody, OTF-11 antibody, PLA-1 antibody, PLA1 antibody, Skn-1a antibody, nrl-16 antibody, nrl16-A antibody, Oct-11a antibody, Oct11 antibody, Otf-11 antibody, Otf11 antibody, Skin antibody, Skin-1a antibody, Skn-li antibody, POU class 2 homeobox 3 antibody, POU class 2 homeobox 3 L homeolog antibody, POU domain, class 2, transcription factor 3 antibody, POU2F3 antibody, pou2f3 antibody, Pou2f3 antibody, pou2f3.L antibody

Background: POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes.Synonyms: OTF-11, OTF11, Oct-11, Octamer-binding protein 11, Octamer-binding transcription factor 11, PLA-1, PLA1, POU domain class 2 transcription factor 3, Skn-1

Gene ID: 25833

NCBI Accession: NP_055167

UniProt: Q9UKI9