Cullin 2 antibody (C-Term)

Catalog No. ABIN498035

Product Details anti-Cullin 2 Antibody

Target: Cullin 2 (CUL2)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Cullin 2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QYIERSQASA DE

Specificity: Recognizes CUL2 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_003582.2 Genename: CUL2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 1-3 μg/mL. Approx 100 kDa band observed in lysates of cell line NIH3T3(calculated MW of 87.0 kDa according to Human NP_003582.2 and of 86.0 kDa according toMouse NP_083678.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cullin 2

Target: Cullin 2 (CUL2)

Alternative Name: Cullin-2 (CUL2 Products)

Synonyms: cul2 antibody, Cullin-2 antibody, fj48a06 antibody, wu:fj48a06 antibody, cullin-2 antibody, LOC100221330 antibody, 1300003D18Rik antibody, 4932411N15Rik antibody, AI327301 antibody, mKIAA4106 antibody, ATCUL2 antibody, F22D16.2 antibody, F22D16_2 antibody, cullin 2 antibody, cullin 2 antibody, cullin-2 antibody, putative cullin-2 (cul-2) antibody, cullin 2 L homeolog antibody, Cullin-2 antibody, cul2 antibody, CUL2 antibody, PTRG_05231 antibody, Smp_079740 antibody, LOC100550749 antibody, Cul2 antibody, cul2.L antibody, cul-2 antibody

Background: Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.Synonyms: CUL-2, CUL2

Gene ID: 8453

NCBI Accession: NP_003582

UniProt: Q13617

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome