GNAS antibody (Internal Region)

Catalog No. ABIN498036

Product Details anti-GNAS Antibody

Target: GNAS (GNAS Complex Locus (GNAS))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This GNAS antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QAARSNSDGE KATK

Specificity: This antibody is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2.

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000507.1, NP_001070956.1, NP_001070957.1. Genename: GNAS

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. Approx 45 kDa band observed in lysates of cell line Jurkat(calculated MW of 45.5 kDa according to NP_000507.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Alternative Name: GNAS / GSP (GNAS Products)

Synonyms: AHO antibody, C20orf45 antibody, GNAS1 antibody, GPSA antibody, GSA antibody, GSP antibody, NESP antibody, PHP1A antibody, PHP1B antibody, PHP1C antibody, POH antibody, GNAS antibody, PORGSA1 antibody, G-alpha-S antibody, 5530400H20Rik antibody, A930027G11Rik antibody, C130027O20Rik antibody, Galphas antibody, Gnas1 antibody, Gnasxl antibody, Gsa antibody, Nesp antibody, Nespl antibody, Oed-Sml antibody, Oedsml antibody, P1 antibody, P2 antibody, P3 antibody, G-alpha-s antibody, gnas-a antibody, Gnpas antibody, Nesp55 antibody, gnal antibody, Gnas antibody, GNAS complex locus antibody, guanine nucleotide-binding protein G(s) subunit alpha antibody, GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus antibody, GNAS complex locus S homeolog antibody, neuroendocrine secretory protein 55-like antibody, GNAS antibody, LOC694289 antibody, LOC469986 antibody, Gnas antibody, gnas.S antibody, gnas antibody, LOC100346323 antibody, LOC100442753 antibody, LOC100732436 antibody

Background: GTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.Synonyms: Adenylate cyclase-stimulating G alpha protein, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Gene ID: 2778

NCBI Accession: NP_000507

UniProt: P63092

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis