ATP1B1 antibody (Internal Region)

Catalog No. ABIN498062

Product Details anti-ATP1B1 Antibody

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP1B1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-KTEISFRPND PKSYE

Specificity: This antibody is expected to recognize both reported isoforms (NP_001668.1, NP_001001787.1).

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of Human ATP1B1 according to NP_001668.1, NP_001001787.1. Genename: ATP1B1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.1-0.3 μg/mL. Detects a band of Approx 48 kDa in Rat Retina lysates and 58 kDa in Mouse and Rat Kidneylysates. The observed molecular weight corresponds to earlier findings with differentantibodies from other commercial sources.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: ATP1B1 (ATP1B1 Products)

Synonyms: ATP1B antibody, Atp4b antibody, Atpb antibody, Atpb-1 antibody, NKbeta1 antibody, ATPBS antibody, atp1b1 antibody, atp1b1a antibody, cb710 antibody, ATPase Na+/K+ transporting subunit beta 1 antibody, ATPase, Na+/K+ transporting, beta 1 polypeptide antibody, ATPase beta chain antibody, ATP synthase CF1 beta subunit antibody, ATPase Na+/K+ transporting subunit beta 1 S homeolog antibody, ATPase, Na+/K+ transporting, beta 1b polypeptide antibody, ATP1B1 antibody, Atp1b1 antibody, atpB antibody, atp1b1.S antibody, atp1b1b antibody

Background: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit a tissue-specific and developmental pattern of expression. The alpha 1 and beta mRNAs are present in all cell types examined whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression.Synonyms: Sodium Pump ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1, Sodium/potassium-transporting ATPase subunit beta-1

Molecular Weight: 35.1 kDa (Human NP_001668.1), 35.1 kDa (Mouse NP_033851.1) and 35.1 kDa (Rat NP_037245.2)

Gene ID: 481

NCBI Accession: NP_001001787

UniProt: P05026

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome