ACAT1 antibody (Internal Region)

Catalog No. ABIN498064

Product Details anti-ACAT1 Antibody

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Binding Specificity: AA 257-269, Internal Region

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Sequence: C-KRVDFSKVPK LKT

Specificity: Recognizes Acetoacetyl Coenzyme A Thiolase/ACAT1 (257-269).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of Human ACAT1 according to NP_000010.1. Genename: ACAT1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.01-0.03 μg/mL. Detects a band of Approx 45 kDa in Human Liver lysates (calculated MW of 45.5 kDaaccording to NP_000010.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1 / MAT (ACAT1 Products)

Synonyms: ACAT antibody, MAT antibody, T2 antibody, THIL antibody, RATACAL antibody, 6330585C21Rik antibody, Acat antibody, fd16h07 antibody, fd20g06 antibody, wu:fd16h07 antibody, wu:fd20g06 antibody, zgc:86832 antibody, acat1-a antibody, acetyl-CoA acetyltransferase 1 antibody, acetyl-Coenzyme A acetyltransferase 1 antibody, acetyl-CoA acetyltransferase 1 L homeolog antibody, ACAT1 antibody, Acat1 antibody, acat1 antibody, acat1.L antibody

Background: ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial

Gene ID: 38

NCBI Accession: NP_000010

UniProt: P24752