MYB antibody (Internal Region)

Catalog No. ABIN498072

Product Details anti-MYB Antibody

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Binding Specificity: AA 557-569, Internal Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-HRDQTVKTQK ENT

Specificity: This antibody is expected to recognize all reported isofoms (NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1, NP_001155132.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide corresponding to internal region of Human c-Myb Genename: MYB

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 85 kDa in lysates of cell line Jurkat.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: MYB (MYB Products)

Synonyms: Cmyb antibody, c-myb antibody, c-myb_CDS antibody, efg antibody, AI550390 antibody, M16449 antibody, cmyb antibody, xmyb antibody, MYB proto-oncogene, transcription factor antibody, myeloblastosis oncogene antibody, MYB proto-oncogene, transcription factor S homeolog antibody, v-myb avian myeloblastosis viral oncogene homolog antibody, MYB antibody, Myb antibody, myb.S antibody, myb antibody

Background: Myb is a transcriptional activator, DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.Synonyms: Proto-oncogene c-Myb, Transcriptional activator Myb, c-Myb

Gene ID: 4602

NCBI Accession: NP_005366

UniProt: P10242

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process